摘要
简述了多维高效液相色谱法的特点及发展简况，重点对分子排阻色谱／反相色谱、离子交换色谱／反相色谱、正相色谱／反相色谱、分子排阻色谱／离子交换色谱、液固色谱／反相色谱、亲合色谱／反相色谱、非手性柱／手性柱等的联用模式及实际应用进行了概括和总结。
MDHPLC is a new chromatographic technique, in which fractions from a separation system are selectively transferred to one or more secondary chromatographic systems with different separation mechanism to enhance resolution and sensitivity, or to decrease analysis times. In this review, the principle, characteristics and developments of MDHPLC are discussed. The various possible combinations of separation modes in HPLC 〔 such as, size exclusion chromatography/reversed phase chromatography (SEC/RPC),ion exchange chromatography/reversed phase chromatography(IEC/RPC), normal bonded phase chromatography/reversed phase chromatography(NPC/RPC), size exclusion chromatography/ion exchange chromatography (SEC/IEC), liquid solid chromatography/reversed phase chromatography (LSC/RPC), affinity chromatography/reversed phase chromatography (AC/RPC) and achiral column/chiral column〕 and their applications are systematically reviewed with 63 references.
出处
《色谱》
CAS
CSCD
北大核心
1997年第6期490-493,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金
关键词
高效液相色谱法
多维分离
分离模式组合
MDHPLC
high performance liquid chromatography, multidimensional separation, combination of separation modes
