摘要
目的观察血管紧张素(Ang)-Ⅱ对血管内皮细胞内皮素(ET)-1 mRNA表达水平的影响及阿托伐他汀的保护作用。方法将培养的血管内皮细胞随机分为4组:空白对照组(仅给予细胞培养液)、单纯Ang-Ⅱ组(细胞培养液中加入Ang-Ⅱ,使其终浓度为10-7mol/L)、Ang-Ⅱ+小剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为0.1μmol/L)、Ang-Ⅱ+大剂量阿托伐他汀组(在单纯Ang-Ⅱ组的基础上加入阿托伐他汀,使阿托伐他汀的终浓度为1μmol/L)。以反转录-聚合酶链反应(RT-PCR)方法检测血管内皮细胞的ET-1mRNA表达水平。结果①与空白对照组相比,单纯Ang-Ⅱ组血管内皮细胞的ET-1 mRNA表达水平显著增高(P<0.01);②与单纯Ang-Ⅱ组相比,两个Ang-Ⅱ+阿托伐他汀组大鼠的心肌ET-1 mRNA表达水平显著降低(P<0.01);③Ang-Ⅱ+高浓度阿托伐他汀组的ET-1 mRNA表达水平显著低于Ang-Ⅱ+低浓度阿托伐他汀组(P<0.05)。结论Ang-Ⅱ可使血管内皮细胞ET-1 mRNA表达水平显著增高,阿托伐他汀可逆转这一作用,且其作用呈剂量依赖性。
Objective To investigate the effects of Ang-Ⅱ on the expression of endothelin （ET）-1 mRNA and the protection effect of Atorvastatin in Vascular Endothelial Cell （VEC）. Methods Cultured VEC were divided into 4 groups： control group （only given cell culture medium） ; Ang-Ⅱ group （given Ang-Ⅱ with the final concentration be 10^- 7 mol/L） ; Ang-Ⅱ ＋ low de, sage atorvastatin group （atorvastatin were given with the final concentration be 0.1 μmol/L at the base of Ang-Ⅱ group; Ang-Ⅱ ＋ high dosage atorvastatin group （atorva.statin were given with the final concentration be 1 μmol/L at the base of Ang-Ⅱ group）. The expression of ET-1 mRNA was evaluated by RT-PCR. Results ①The expression level of ET-1 mRNA in Ang-Ⅱ group is significantly higher than that of control group （P 〈 0.01 ） ; ②The expression level of ET-1 mRNA in Ang-Ⅱ ＋ atorvastatin group is significantly lower than that orang- Ⅱ group （ P〈0.01 ） ; ③The exprexsion level of ET-1 mRNA in Ang-Ⅱ ＋ high dosage atorvastatin group is significantly lower than that of Ang- Ⅱ ＋ low dosage atorvastatin group （ P 〈 0.05）. Conclusion Ang-Ⅱ increases the expression of ET-1 mRNA in VEC, Atorvastatin inhibits the expression of ET-1 mRNA in a dose-dependent manner.
基金
海军后勤科研基金资助项目(00-3321)
