摘要
目的探讨与细胞周期调节有关的基因改变在食管鳞状细胞癌中的表达及其意义。方法应用免疫组织化学链霉抗生物素蛋白过氧化物酶连接法（ＳＰ法），检测７３例食管癌中Ｒｂ蛋白和ｐ１６蛋白的表达。结果食管鳞状细胞癌Ｒｂ和ｐ１６蛋白表达阳性率分别为４２５％（３１／７３）和６７１％（４９／７３），Ｒｂ蛋白表达阳性率与食管鳞状细胞癌分级呈显著负相关（Ｐ＜０００５），ｐ１６蛋白表达阳性率与食管鳞状细胞癌分级呈显著正相关（Ｐ＜０００５）。４９例ｐ１６蛋白表达阳性的食管癌中Ｒｂ蛋白表达阴性者３９例，３１例Ｒｂ蛋白表达阳性的食管癌中ｐ１６蛋白表达阴性者２１例，两者呈显著负相关（Ｐ＜０００５）；１０例食管鳞状细胞癌呈Ｒｂ蛋白和ｐ１６蛋白共同表达，３例Ｒｂ蛋白和ｐ１６蛋白表达呈共同阴性。结论Ｒｂ蛋白和ｐ１６蛋白表达的有无可分别作为食管鳞状细胞癌病理分级参考指标之一，并间接证明Ｒｂ蛋白对ｐ１６蛋白表达具有负性调节的功能。
PURPOSE To find the expression and significance of gene changes which were related to cellcycle regulator.METHODS In 73 esophageal squamous cell carinomas, the immunohistochemical method was used to study the expression of Rb and p16 proteins.RESULTS Rb and p16 positive rates were 425%(31/37) and 671%(49/73) respectively.For Rb the positive rates decreased with the progression of the pathological grade (P<0005),while for p16 the positive rates increased (P<0005).39/49 p16positive specimens did not stain for Rb,21/31 Rb positive specimens did not stain for p16,there was thus an inverse relationship between the expression of Rb and p16 protein (P<0005).10 cases were Rb and p16 double positives,and 3 cases were Rb and p16 double negatives.The presence or absence of expression of Rb and p16 protein is one of the reference indices for pathological grading in esophageal squamous cell carcinoma.CONCLUSIONS Our results indirectly support the theory that p16 expression is negatively regulated by the functional Rb protein.
出处
《中国癌症杂志》
CAS
CSCD
1997年第4期276-279,共4页
China Oncology
关键词
食管肿瘤
鳞状细胞癌
P16
蛋白
免疫组化
esophageal neoplasms
squamous cell carcinoma
retinoblastoma protein
p16 protein
immunohistochemistry
