摘要
１０羽１４日龄鸡分别以马立克氏病毒（ＭＤＶ）ＢＪ－１株接毒，采集羽毛根作为ＭＤＶ琼扩抗原和ＭＤＶＤＮＡｄｏｔ－ｂｌｏｔ杂交的动态检测样品。接毒后第１０ｄ至第８周的检测结果显示，鸡羽毛根ＭＤＶ琼扩抗原及ＭＤＶＤＮＡ可分别在接毒后第１４ｄ（３７．５％，３／８）和第１２ｄ（３３．３％，３／９）检出；接毒后第１７ｄ至第５周，鸡羽毛根ＭＤＶ琼扩抗原的阳性检出率均大于８０％；第６，７和８周时，则分别降至６６．７％（４／６），５０％（３／６）和４０％（２／５）。而ＭＤＶＤＮＡ的检测，除１只鸡直到第４周才呈阳性外，其余在接毒后第１４ｄ至第８周中均保持１００％的阳性检出率。
The 14oldday chickens were inoculated with Marek′s disease virus (MDV) strain Beijing1 (BJ1). During 10 days to 8 weeks postinjection (PI), a detection of MDV antigens and DNA in the feather tips from same injected chickens were made using two assays: agargelprecipitation (AGP) and dotblot DNA hybridization. The results showed MDV in the feather tips were identified 14 days PI (37.5%, 3/8) by AGP and 12 days PI (33.3%, 3/9) by DNAhybridization. Following detection, positive rates of MDV were always above 80% from 17 days to 5 weeks PI by AGP and were decreased significantly to 66.7% (4/6), 50%(3/6) and 40%(2/5) at week 6,7 and 8 PI by AGP, respectively. However, the exception of one bird was negative since week 4 PI. the positive rates were always 100% from 14 days to 8 weeks PI by DNAhybridization.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第4期75-78,共4页
Journal of Nanjing Agricultural University
关键词
鸡病
羽毛根
马立克氏病
病毒
检测
DNA
chicken
feather tips
MDV
detection
AGP
DNA dotblot hybridization
