摘要
[Objective]To clone and identify Marek's disease virus(MDV)serum gene.[Method]MDV genomic DNA was extracted from lymphoid tissues of the diseased chickens with latent MDV infection.The MDV L-meq gene was amplified by gradient PCR,inserted into pMD18-T vector and sequenced.The sequence was analyzed using DNAman software.[Result]The obtained sequence had 100%similarity with the published se-quence of L-meq gene,showing successful amplification of target gene.[Conclusion]The paper provides new ideas and new methods for preven-tion and treatment of Marek's disease in chickens.
基金
funded by the grants of Jilin Provincial Department of Education(2010 No.382)
Natural Science Foundation of Changchun Normal University(2010 No.023)
