摘要
目的:探讨RNA编辑酶ADAR1的表达受抑制后,小鼠淋巴细胞的细胞周期和细胞凋亡的变化.方法:用电转法将ADAR1特异性siRNA转入到处于混合培养的小鼠淋巴细胞中,培养48h,RT-PCR检测转染效率;流式细胞仪检测细胞周期和凋亡变化;RT-PCR检验周期蛋白D1(cyclin D1)基因和周期蛋白A1(cyclin A1)基因表达量的变化.结果:转染ADAR1特异性siRNA48h后,小鼠淋巴细胞G0/G1期细胞量增加,S期细胞量减少,G2/M细胞量保持恒定.另外,cyclinD1基因的表达量降低而cyclin A1基因表达保持恒定.结论:处于经典混合培养体系下的小鼠淋巴细胞,在其RNA编辑酶ADAR1受到特异siRNA抑制后,小鼠淋巴细胞的周期受到明显抑制,细胞凋亡增加.
AIM： To investigate the effect of ADAR1 suppression on the cycle and apoptosis of mixed cultured lymphocytes. METHODS： ADAR1 specific siRNA was transfected into mouse lymphocytes by electroporation. After 48 h, the ratio of transfection was tested by RT-PCR. The cell cycle and apoptosis were detected by flow cytometry. The expression of mRNA of cyclin D1 gene and A1 gene were detected by RT-PCR. RESULTS： At 48 h after the transfection, the number of lymphocytes in the G0/G1 phase was increased, that in the S-phase was depressed, and that in the G2/M phase was constant. The expression of cyclin D1 gene was decreased, and that of cyclin A1 gene kept constant. CONCLUSION： Cell cycle is inhibited and the apoptosis is increased when ADAR1 is restrained by the specific slRNA in mixed cultured mouse lymphocytes.
出处
《第四军医大学学报》
北大核心
2008年第17期1561-1564,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30271281
30170923)
