摘要
目的:探讨干扰乙醛脱氢酶1A1(ALDH1A1)基因表达后对鼻咽癌5-8F和CNE2细胞生长、增殖的影响。方法:构建ALDH1A1 siRNA表达质粒稳定转染鼻咽癌5-8F和CNE2细胞,Western blot检测干扰ALDH1A1基因蛋白表达水平。MTT、平板克隆、裸鼠成瘤实验检测干扰ALDH1A1基因前后鼻咽癌5-8F和CNE2细胞的增殖、克隆及成瘤能力。结果:与空白组和阴性对照组相比,干扰ALDH1A1表达后5-8F和CNE2细胞增殖、克隆及肿瘤形成能力明显受到抑制。结论:干扰ALDH1A1基因表达,可抑制鼻咽癌细胞的生长、增殖、克隆及成瘤能力。
Objective：To explore the growth and proliferation of nasopharyngeal carcinoma （NPC） 5-8F and CEN2 cell lines by the expression of suppressed ALDH1A1 gene.Methods：ALDH1A1 specific siRNA expression plasmid and non-specific siRNA expression plasmid were constructed and transected in 5-8F and CNE2 cells.The protein level of ALDH1A1 was assessed by Western blot.We assayed the proliferation and tumorigenesis of ALDH1A1 in vitro and vivo.Results：5-8F and CNE2 cells grew slower and had lower clone formation efficiency and had lower tumorigenesis than control and untreated cells after suppressed ALDH1A1 gene in vitro and vivo.Conclusion：ALDH1A1 downregulation inhibits cell proliferation,clone formation efficiency and tumorigenesis in nasopharyngeal carcinoma cells.
出处
《现代肿瘤医学》
CAS
2014年第2期269-272,共4页
Journal of Modern Oncology
基金
国家青年科学基金资助项目(编号:81201672)
湛江市科技攻关计划项目(编号:2012C3104032)
