摘要
目的探讨猪食管肌间神经节内垂体腺苷酸环化酶激活肽(PACAP)免疫阳性神经末梢的来源。方法采用荧光免疫组织化学技术及逆向神经元示踪技术。结果猪食管肌间神经节内含有叶片状、PACAP免疫阳性的神经纤维网;经食管下括约肌腹侧壁行荧光素快蓝(FB)神经元逆向示踪研究显示,节神经节(NG)内含有许多FB示踪神经元,其中约有48%呈PACAP免疫阳性。结论猪食管肌间神经节内的PACAP免疫阳性神经末梢网很可能来源于迷走神经,为猪食管壁内的肌间神经节内板层样神经末梢。NG神经元顺向示踪研究结合免疫组织化学技术,将有助于确定此结构的形态特点并揭示其神经化学特征。
Objective To study the origin of PACAP-immunoreactive nerve fibers in myenteric ganglia of porcine esopha gus. Methods Immunohistochemistry combined with retrograde neuronal tracing technique with fast blue was applied. Results Dense leaflet-like PACAP immunoreactive nerve fibers were found in the myenteric ganglia of porcine esophagus, especially in the region of abdominal wall of lower esophageal sphincters; many fast blue-traced neurons were found in nodose ganglia, among which, about 48% were PACAP immunoreaetive. Conclusion The dense PACAP immunoreaetive nerve fibers found in the my enteric ganglia of porcine esophagus were possibly the intraganglionic lamina endings of vagal origin.
出处
《青岛大学医学院学报》
CAS
2008年第4期347-348,351,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅科研基金资助项目(2005HZ017)
关键词
食管
神经节
神经末梢
免疫组织化学
猪
Esophagus
Ganglia
Nerve endings
Immunohistochemistry
Swine
