摘要
目的探讨进展期胃癌核因子-κB(nuclear factor-kappa B,NF-κB)蛋白的表达,上皮钙黏蛋白(E-cadherin,E-cad)及β-catenin在转移淋巴结内癌细胞中的再表达情况及其与临床病理因素的关系。方法用免疫组织化学染色法检测NF-κB在原发灶和E-cad及β-catenin在86例进展期胃癌原发灶及其相应转移淋巴结内癌细胞的表达情况。结果胃癌原发灶中81.40%(70/86)出现NF-κB阳性,46.51%(40/86)转移淋巴结内癌细胞中出现E-cad及β-catenin再表达阳性,NF-κB与肿瘤的分化程度、病理类型密切相关(P<0.05),E-cad及β-catenin在转移淋巴结内的再表达阳性与肿瘤的分化程度密切相关(P<0.05),E-cad在转移淋巴结内的再表达与肿瘤的病理类型密切相关(P<0.05),β-catenin在转移淋巴结内的再表达与肿瘤的病理类型无关(P>0.05),NF-κB的表达与E-cad及β-catenin在转移淋巴结内癌细胞中的再表达情况与肿瘤的大小及浸润程度无关(P>0.05)。E-cad及β-catenin在转移淋巴结内再表达存在相关关系(P<0.05),NF-κB在肿瘤原发灶的表达与E-cad及β-catenin在转移淋巴结内的再表达无相关关系(P>0.05)。结论NF-κB的表达与进展期胃癌的发展过程密切相关,E-cad及β-catenin在转移淋巴结中的再表达是进展期胃癌发生的重要事件,二者协同发挥作用,并对癌细胞在转移灶中的聚集生长起重要作用,联合评价其功能具有更重要的临床意义。
Objective The aim of this study was to investigate the expression of nuclear factor-kappa B（NF-κB） in the primary cancer cells and the re-expression of E-cadherin and β- catenin in cancer cells of metastatic lymph nodes in advanced gastric carcinoma. Methods The expression of NF-κB and E-cadherin,β-catenin was detected immunohistochemically in 86 primary gastric tumor and the corresponding matastatic lymph nodes. Results Among the 86 primary gastric carcinoma,the expression of NF-κB was detected in 70 cases（81, 40%）. The expression of NF-κB was closely related to pathological differentiation and pathological types. As re-expression of E-cadherin and β-catenin in metastatic cancer cells compared with their primary tumors was detected in 40 cases from 86 cases with lymphynode metastatics （46.51%）, and the re-expression Of E-cadherin and β-catenin in metastatic lymph nodes was more frequently detected in the well or moderate differenciated tumors than in that in the poor differenciated tumors, The re-expression of E-cadherin in metastatic lymph nodes was closely related to tumor pathological types. Conclusion The expression of NF-κB protein, is related to progression of the advanced gastric carcinoma. The re-expression of E-cadherin and β-catenin in cancer cells of metastatic lymph nodes from advanced gastric carcinoma existed,and which was closelY correlated with tumor grade,may play an important role in the re-growth of cancer cells in metastatic loci.
出处
《河北医科大学学报》
CAS
2008年第5期647-651,801,共6页
Journal of Hebei Medical University
基金
河北省自然基金资助项目(NO.C2005000762)
关键词
胃肿瘤
淋巴结
免疫组织化学
stomach neoplasms
lymph nodes
immunohistochemistry
