摘要
[目的]对1个水稻生物产量突变体进行遗传分析。[方法]对转Bar基因水稻后代的筛选和鉴定的过程中,在T1代群体10个株系中发现1个生物产量突变株系;采用除草剂Basta喷洒及PCR扩增等方法对该株系进行遗传分析并进行农艺性状考察。[结果]该基因突变除了使株型增高外,径秆也较粗,开花较早,分蘖多,穗大,生物产量高。该株系分离比率为3∶1,符合孟德尔遗传分离规律。PCR分子检测证实,突变性状与T-DNA插入的目的基因(Bar)没有共分离,表明突变体不是由目的基因的T-DNA插入所引起的。[结论]该研究有助于克隆该突变体诱发基因及理解其对生物产量影响机制。
[ Objective ] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa. [Method] In the process of screening and identification of Bar-transgenic rice,a biomass mutant was found in 10 lines of T1 progenies. The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification. [Result] The segregation ratio is consistent with mendelian law（3： 1）.The mutant assumes not only higher plant height, wider straw and earlier florescence, but also more tillers, bigger spikes and resnltanfly higher biomass. PCR detections indicate that no co-segregation was observed between mutant traits and target gene （Bar） of T-DNA inserted,proving that the mutant is not caused by the insertion of T-DNA containing target gene （ Bar）. [ Conclusion] Our study may avail to understand the Cloning of mutant gene and the mechanism of the mutant gene on biomass.
出处
《安徽农业科学》
CAS
北大核心
2008年第22期9451-9452,9626,共3页
Journal of Anhui Agricultural Sciences
关键词
水稻
生物产量
突变体
遗传分析
Oryza sativa
Biomass
Mutant
Genetic analysis
