摘要
建立一种测定豆浆中游离氨基酸的反相高效液相色谱法。优化条件为:Eclipse XDB—C_(18)反相柱(150mm×4.6mm,5μm);流动相:甲醇和0.05mol/L醋酸钠的体积比线性梯度(T,B%)=0,0%;20,20%;50,90%,流速:1.0mL/min,检测波长:338nm。在此条件下得到各游离氨基酸的直线回归方程,相关系数都在0.9900以上。豆浆煮制过程中游高氨基酸含量逐步增加,回收率在90.86%～106.05%之间。
In this paper, a method of reverse high performance liquid chromatography for determina the free amino acids in soybean milk was established. The optimized chromatographic condition were as ting followed： Eclipse XDB-C18 stainless column （150mm×4.6mm,5μm） was used. The mobile phase was as follows： V （methanol） ：V（0. 05mol/LNaAC （T,B%）-0.0% ;20.20%;50.90%,and the flow rate was 1.0 mL/ min. It was measured by an ultraviolet detector under 338 nm. The coefficients of linear equations of 18 free amino acid were all above 0. 9900. The free amino acids in soybean milk increased with the increasing of the time of boiling. The recoveries were 90.86%-106.05%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第7期146-149,共4页
Food and Fermentation Industries
基金
广东省科技攻关资助项目(No.200482041003)
关键词
HPLC
豆浆
游离氨基酸
测定
HPLC, soybean milk, free amino acids, detection
