摘要
目的建立葛根及粉葛化学成分谱高效毛细管电泳分析方法。方法采用HPCE/DAD分析技术,缓冲液为含16.7%甲醇的40mmol/L硼砂,压力进样:137.9kPa,5s;分离电压:0～5min,25kV;5～25min:22kV;毛细管温度:20℃。结果建立了葛根及粉葛化学成分谱的HPCE分析方法,鉴定了葛根素、大豆苷元两种主要成分。结论本方法是一种简单、快速,可用于葛根及粉葛指纹图谱研究的方法。
Objective To develop a method for analyzing the chemical compositions of Gegen and Fenge extracts using high-performance capillary electrophoresis （HPCE）. Methods Using HPCE/DAD, the chemical composition of the extracts was analyzed with the buffer solution of 40 mmol/L borax containing 16.7% methanol, with injection pressure at 137.9 kPa for 5 s, separation voltage at 25 kV in 0-5 min time range and at 22 kV in 5-25 min time range, and the temperature of the capillary of 20 ℃. Results and Conclusion The method for analysis of Gegen and Fenge extracts was established, which identified puerarin and daidzein as the two major components. This simple and rapid analysis method can be used for Gegen and Fenge extract fingerprinting.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第8期1407-1408,共2页
Journal of Southern Medical University
基金
国家自然科学基金(30500668)
关键词
葛根
粉葛
化学成分谱
高效毛细管电泳
Gegen
Fenge
chemical composition electropherogram
high-performance capillary electrophoresis
