摘要
用聚丙烯酰胺凝胶电泳技术对荞麦属（Fagopyrum Mill）8个种（含大粒组7个种和小粒组1个种）29份栽培及野生荞麦植株三叶期幼叶的酯酶同工酶进行了研究。结果发现：酯酶同工酶酶带共31条，不同物种的酶带数4～9条，其中甜荞有8条带，而苦荞为9条。酶带分析及聚类分析表明：大粒组荞麦种的谱带与细野荞（F．gracilipes）等小粒组荞麦种间差异极大，甜荞（F.esculentum）和苦荞（F．tataricum）酶带分别与大野荞（F.megaspartanium）和毛野荞（F.pilus）相似，并分别与大野荞和毛野荞聚类最近，提示大野荞和毛野荞可能分别是甜荞和苦荞的祖先种。
The esterase isozymes of twenty-nine accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum were studied by means of polyacrylamide gel eletrophoresis （PAGE）. The results showed that there are a total of thirty-one bands of esterase isozymes, with a range from four bands to nine bands among different buckwheat species. F. esculentum and F. tataricum respectively have a total of eight bands and nine bands among different accessions. The results of the zymograph analysis and the clustering showed that there are much great difference of zymographs between the big-achene group and the small-achene group and that F. megaspartanium and F. pilus are close in clustering tree to F. esculentum and F. tataricum, respectively, supporting Chen＇s hypothesis that F. megaspartanium and F. pilus may be the ancestors of F. esculentum and F. tataricum, respectively.
出处
《武汉植物学研究》
CSCD
北大核心
2008年第4期428-432,共5页
Journal of Wuhan Botanical Research
基金
国家自然科学基金项目(30471116)
教育部新世纪人才支持计划(NECT2004-0931)
国家“十一·五”科技支撑计划(2006BAD02B06)
贵州省动植物品种专项(黔农育专字[2007]026号)
贵州省教育厅自然科学培育项目(黔教科2006103)
关键词
甜荞
苦荞
野荞
酯酶同工酶
系统关系
Common buckwheat
Tartary buckwheat
Wild buckwheat
Esterase isozyme
Phylogeny
