摘要
将应用常规RPHA法检查HBsAg阴性的1074份献血员血清再用ELISA和SPRIA法检测。其中又查出HBsAg、HBeAg和抗-HBc-IgM标志,其阳性率分别达5.96％,2.14％和3.35％,HBV携带率竟高达9.68％。说明用常规RPHA法筛选出的HBsAg阴性献血员仍有传播乙型肝炎的危险。用ELISA法并联检测HBsAg和抗-HBc-IgM,在RPHA法检查HBsAg阴性的献血员中、其阳性率为8.29％,敏感度达85.58％,因而这种并联筛检方法可成为一种切实可行的筛检方法。
Cryopreseved sera of 1074 blood donors that had proved HBsAg negative by RPHA were reexam-ined for HBsAg HBeAg and anti-HBc-IgM by ELISA and SPRIA and donors residue sera after ELISA, with either HBsAg, HBeAg, or anti-HBc-IgM positive had a test for HBV-DNA with spot hybridization. The positive rate of HBsAg, HBeAg, anti-HBc-IgM and HBV-DNA were 5. 96%, 2. 14% , 3. 35% and 5. 40% respectively. As ELISA screen on each marker had sensitivities below 70% ,and parallel of two markers can increase the sensitivity; parallel of HBsAg and anti-HBc-IgM, sensitivity reached up to 85. 58% ,it is rather practicable. The RPHA detection of HBsAg have a very high risk of transmission of HBV,that should be refused.
出处
《湖南医科大学学报》
CSCD
1990年第4期347-349,共3页
Bulletin of Hunan Medical University
关键词
乙型肝炎
传播
输血
预防
transmission
hepetitis B virus
blood transfussion
prevention and control 1)Sanitation and anti-epidemic station of Handan city,Heber
