摘要
农杆菌介导将美洲商陆抗病毒蛋白基因(pap)导入烟草云烟87品种的叶肉细胞,经过抗性筛选和鉴定,获得转基因植株.利用RT-半定量法分析了Y1、Y2和Y3转基因株系的pap表达,以及攻毒前后叶片多酚氧化酶(PPO)、超氧物歧化酶(SOD)活性和可溶性蛋白质含量.结果表明:pap在Y1和Y2植株中表达,Y3植株中未检测到pap表达;攻毒后,Y1和Y2叶片中可溶性蛋白质含量、PPO和SOD活性较高,而Y3叶片中可溶性蛋白质含量、PPO和SOD活性在三者中最低,与对照相似.讨论了转基因植株中pap表达与其生理变化和病毒抗性的关系.
Mesophyll cells from Nicotiana tabacurn var. Yunyan 87 was transfected with pokeweed antiviral protein （pap） gene by Agrobacteriurn turnefaciens, transgenic plants were obtained by screening on Km-containing medium and confirmed with PCR. pap expression in three transgenant clones Y1, Y2 and Y3 was determined by semi-quantitative method, and activities for superoxide dismutase （SOD）, polyphenol oxidase （PPO） and contents of soluble protein are analyzed before and after infection with tobacco mosaic virus （TMV）. pap expression was found in clones Y1 and Y2, but not in Y3. After TMV infection, soluble protein content and activity for SOD and PPO were found to be higher in clones Y1 and Y2 than Y3, Y3 levels being similar to that in wide-type. It is therefore concluded that pap expression in transgenic plants is correlated with physiological changes and viral-resistance.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第4期411-416,共6页
Journal of Beijing Normal University(Natural Science)
关键词
商陆抗病毒蛋白
烟草
多酚氧化酶
超氧物歧化酶
可溶性蛋白质
pokeweed antiviral protein
Nicotiana. tabacum
polyphenol oxidase
superoxide dismutase
soluble protein
