摘要
将带有启动子GaMV35S、终止子Nos ter的雪花莲凝集素基因GNA插入到已构建好的植物表达载体pBI121/α PAP上,构建成双抗表达载体pBI121/GNA+α PAP,并采用农杆菌介导法将其转化进入烟草红花大金元,获得了卡那霉素筛选的抗性植株,通过PCR扩增验证,烟草基因组中含有这两个抗性基因。
A plant expression vector with resistance to both virus and aphid was constructed by inserting Galanthus nivalis agglutinin gene(GNA)containing CaMV35S promoter and Nos terminal into pHI121／αPAP,a Pokeweed Antiviral Protein gene.GNA gene and αPAP gene were transferred into tobacco mediated by Agrobacterium tumefaciens and under Kanamycin selection pressure.The results of PCR from the kanamycin resistant transformants indicated that GAN gene and αPAP gene have been integrated into the tobacco genome.
出处
《西南农业学报》
CSCD
2002年第4期35-37,共3页
Southwest China Journal of Agricultural Sciences
基金
云南省应用基础研究(97C001G)
