摘要
用碱水解后再用乙醇分级分离,从地龙中获得抗凝血酶活性。该活性不能被AT—Ⅲ抗体及鱼精蛋白中和,表明其活性表达不依赖于AT—Ⅲ。活性提取液在216nm处有单一吸收峰,费林试验阴性,双缩脲试验阳性,提示该活性不属多糖或蛋白质,而可能是一种耐碱耐热的小肽或含双键化合物,与肝素及其类似物、水蛭素等抗凝物质不相同。
By means of a procedure of alkaline hydrolysis followed by alcohol fractiona - tion, an antithrombin activity was obtained from earthworm. Both anti-AT-Ⅲ antibodies which almost completely abolished the antithrombin effect of heparin and protami n could not quench the abstracted antithrombin activity, suggesting in the nature of AT-Ⅲ independent. The solution containing the antithrombin activity showed a single absorption peak at 216nm, a positive biuret reaction and, after acid hydrolysis, a negative reaction with Fehling's reagent. These results indicate that the abstracted antithrombin actvity is neither polysaccharide nor protein and is different from the other anticoagulants known so far.
出处
《湖南医科大学学报》
CSCD
1990年第2期112-114,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
地龙
抗凝活性
提取液
oligochaeta
antithrombins
isolation and purification
drug effect
