摘要
以南方红豆杉(Taxus chinensis var.mairei Cheng et L.K.Fu)的营养器官嫩茎、叶和叶柄等为外植体进行愈伤组织诱导研究,试验结果表明:(1)各外植体中诱导愈伤组织的难易程度为叶柄处>叶片>茎段;(2)2,4-D比NAA更有利于愈伤组织的形成;(3)B5比MS培养基更有利于愈伤组织的形成,因此诱导南方红豆杉产生愈伤组织的最佳培养基配方是:B5+2,4-D4.0mg·L^(-1)+ABT生根粉0.3 g·L^(-1),愈伤组织的诱导率达93%;(4)继代增殖培养的配方B5+2,4-D0.1 mg·L^(-1)+ABT生根粉0.1 g·L^(-1),增殖系数可达3.6.
In this experiment, various explants - tender shoots, leaves and stalk derived from the Taxus （ chinensis var. mairei Cheng et L. K. Fu） had been used to induce the callus. The result showed that： （1）for inducing the callus,the optimized tissue were the leaves stalks , secondly were the leaves, finally the egments of tender shoots; （2） the optimum forum for inducing callus was： B5 ＋ 2,4 - D4.0 mg·L^-1 ＋ ABT0.3 mg·L^-1 . （3）B5 cultural medium was more effective than MS; （4）for subculture , B5 ＋ 2,4 - D0. 1 ＋ ABT0. 1mg·L^-1 could increase the propagational coefficient by 3.6.
出处
《韶关学院学报》
2008年第3期69-72,共4页
Journal of Shaoguan University
关键词
南方红豆杉
外植体
组织培养
愈伤组织
Taxus chinensis var. rnairei Cheng et L. K. Fu
explants
tissue culture
callus
