摘要
以Nipponbare(japonica)/Kasalath(indica)//Nipponbare BC1F10的98个家系为材料,在3种环境下对水稻粒形相关性状及千粒重进行数量性状基因位点(quantitative trait loci,QTL)的定位分析。利用QTL mapper 1.6软件在全基因组水平上检测了粒形相关性状及千粒重QTL。结果表明,在3种环境下共检测到4、1、4、5、5个QTL,分别控制粒长、粒厚、粒宽、粒形和千粒重。鉴定了以Nipponbare为背景、Kasalath为置换片段的染色体片段置换系群体在3种环境中的表型值,发现与背景亲本相比,置换片段包含qGL-6的株系NK28、包含qGL-12的株系NK32粒长均明显变长;置换片段包含qGW-1和qGW-7的株系NK9和NK32粒宽均明显变窄;置换片段包含qLWR-5的株系NK22和NK32、包含qLWR-12的株系NK32粒形明显变大;置换片段包含qTGW-1-1的株系NK9千粒重显著降低。上述结果表明,这些QTL是稳定表达的主效QTL。
Ninety-eight backcross inbred lines derived from Nipponbare （japonica） /Kasalath （indica） //Nipponbare were used to map QTL for grain shape related traits and 1 000-grain weight under the three environments. By QTL mapper 1.6 software, QTL controlling grain shape related traits and 1 000-grain weight were detected on the whole genome. The number of QTL for grain length, grain thickness, grain width, the ratio of grain length to width, and 1 000-grain weight were 4, 1, 4, 5 and 5, respectively. Further stability analysis was conducted using chromosome segment substitution lines （CSSL） with Nipponbare as the genetic background and Kasalath as the donor in three environments. The results showed that significant difference of phenotypic values existed between the recurrent parent, Nipponbare, and the NK28 harboring qGL-6, NK32 harboring qGL-12 alleles. Compared to Nipponbare, the seeds of NK28 and NK32 became longer. The two CSSL both harboring qGW-1 and qGW-7 allele, including NK9 and NK32, had markedly decreased in grain width across the three environments. The same case was for the two CSSL harboring qLWR-5 allele, namely NK22 and NK32, as well as the CSSL NK32 harboring qLWR-12 allele, and the CSSL NK9 harboring qTGW-1-1 allele. These effects of the alleles at above loci were significant and stable. The results provided the foundation for further map-based cloning and marker-assisted selection （MAS） breeding.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2008年第3期1-7,共7页
Journal of Nanjing Agricultural University
基金
国家863计划项目(2006AA10Z1A5)
国家科技攻关计划(2006BAD13B01,2006BAD01A01-5)
江苏省水稻种质资源基因库建设项目(SX(2007)g02)
长江学者和创新团队发展计划
关键词
水稻
粒形相关性状
千粒重
QTL
稳定性分析
Oryza sativa L.
traits related to gain shape
1000-grain weight
QTL
stability analysis
