摘要
为了给河北地区预防和控制猪繁殖与呼吸综合征（PRRS）提供理论数据，根据GenBank公布的PRRSV CH-1a株和JXA1株的核苷酸序列，设计并合成三对特异性引物。应用RT-PCR方法分别扩增PRRSV HB-4（hs）株的ORF5，ORF6，ORF7基因片段。分别将片段克隆入pGmT载体后进行测序，并与多株GenBank中发表的PRRSV毒株ORF5—7基因的核苷酸序列进行比较和变异分析。结果表明，PRRSV HB-4（hs）株属于美洲型，其与近两年流行的毒株群相似性高达98．8％～99．5％；与早些年流行毒株群相似性较低，为91．9％～92．6％。系统进化树表明：HB-4（hs）株及近两年流衍的毒株可能均由早期分离的河北HB-1（sh）株进化而来。
In order to offer the theoretical data for prevent and control PRRS that unparalleled outbreak in Hebei. According to the reported complete nucleotide sequence of PRRSV CH-1a and JXA1 in C, eneBank ,3 pairs of primers were designed to amplify ORF5-7 gene of HB-4（hs） strain with Primer 5.0 software. The ORF5-7 gene nucleotide sequences were obtained by RT-PCR and sequenced after being cloned directly into the pGM-T vector. The obtained sequences were analyzed and compared with the published complete nucleotide sequences of other strains in GeneBank by DNAstar software.The results showed that the PRRSV HB-4（hs） strain was clustered within North American genotopy.The ORF5-7 gene of HB-4（hs） strain showed highest homology of 98.8% -99.5% to the strains were isolated in recent years,showed 91.9% -92.6% identity with the strains were isolated in last years.The phylogenetic trees revealed the strains were isolated in recent years had close relationship with HB-1（sh） strain.
出处
《华北农学报》
CSCD
北大核心
2008年第4期32-36,共5页
Acta Agriculturae Boreali-Sinica
基金
河北省重大技术创新项目资助(07221008Z)
关键词
猪繁殖与呼吸综合征病毒
ORF5—7基因
遗传变异分析
Porcine reproductive and respiratory syndrome virus
ORF5-7 gene
Heredity and variation analysis
