摘要
目的:观察盐酸氯丙嗪与紫杉醇联合应用对人喉癌细胞株(Hep-2细胞)的增殖抑制作用及其对细胞周期各时相的影响。方法:对数生长期Hep-2细胞分为单纯紫杉醇组(3.0、6.0和12.0mg·L-1)、氯丙嗪4.0mg·L-1)加紫杉醇(12.0mg·L-1)联合组和空白对照组(100mL培养液),应用MTT比色法和流式细胞术检测各组Hep-2细胞的增殖抑制率,流式细胞仪分析单独及联合用药后Hep-2细胞的周期进程及凋亡率。结果:3.0、6.0和12.0mg·L-1单纯紫杉醇组Hep-2细胞增殖抑制率分别为14.0%、23.9%和36.7%,随紫杉醇浓度增加细胞增殖抑制率升高,三组间及组间两两比较差异均具有显著性(P<0.05);联合组Hep-2细胞增殖抑制率为46.3%,明显高于相同剂量单纯紫杉醇组(P<0.05);单纯紫杉醇组随其用药浓度的增加G1期细胞减少,S期和G2期细胞增多,与空白对照组比较凋亡率升高(P<0.05)。结论:在一定浓度下,盐酸氯丙嗪能够增强紫杉醇诱导Hep-2细胞增殖抑制率及细胞周期进程各时相变化和凋亡率,氯丙嗪与紫杉醇联合应用具有明显的协同效应。
Objective To investigate the proliferation inhibition of chlorpromazfne combined with taxol on Hep-2 cells （human laryngeal carcinoma cell line） and the effects on cell cycle progression. Methods Hep-2 cells at logarithmic growth phase were divided into taxol groups （3.0, 6.0 and 17,. 0 mg·L^-1 ）, chlorpromazine （12.0 mg·L^-1） comined with taxol （4 mg·L^-1） group and control group （100 mL culture fluid）. MTT and flow cytometry were used to detect the proliferation inhibition rates of Hep-2 cells in various groups. Flow cytometry was also used to analyze the cell cycle progression of Hep-2 cells and apoptotic rate after administration. Results The proliferation inhibition rates in 3.0, 6.0 and 17,. 0 mg·L^-1 taxol groups were 14.0%,23.9% and 36. 7%, respectively, there were significant differences between three groups （P 〈0. 05）; the proliferation inhibition rate in combined group （46.3%） was higher than those in taxol groups （P〈0.05）. In taxol groups. the number of G1 phase cells decreased and the number of S and G2 phase cells increased with the increasing of concentration of taxol. Compared with control group, the apoptotie rates in taxol groups increased （P〈0.05）. Oonclusion Within certain concentration range, chlorpromazine could enhance taxol-induced proliferation inhibition rate, cell cycle progression and apoptotic rate of Hep-2 cells, chlorphomaxine and taxol have significant cooperation effect.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第1期124-126,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(20030550-4)
