摘要
为了探讨小儿病毒性肝炎发病的细胞免疫机制，应用不标记抗体桥联酶标技术（ＡＰＡＡＰ法）检测了５６例病毒性肝炎患儿的外周血Ｔ细胞亚群及膜性白细胞介素２受体（ｍＩＬ－２Ｒ）表达率。应用酶联免疫吸附（ＥＬＩＳＡ）法分别检测了４９例病毒性肝炎患儿单个核细胞培养上清液（ＰＢＭＣｓ）中肿瘤坏死因子α（ＴＮＦα）、白细胞介素６和８（ＩＬ－６、ＩＬ－８）及α干扰素（ＩＦＮ－α）浓度。结果显示：甲型肝炎（简称甲肝）急性期、慢性乙型肝炎（简称乙肝）及丙型肝炎（简称丙肝）组与正常对照比较，ＣＤ４细胞降低，ＣＤ８细胞升高。用植物血凝素（ＰＨＡ）刺激前，甲肝急性期患儿ｍＩＬ－２Ｒ表达率明显高于其他各组，用ＰＨＡ刺激后，各组ｍＩＬ－２Ｒ表达率均显著高于刺激前，但乙肝及丙肝组ｍＩＬ－２Ｒ表达率与正常对照比较则明显降低。甲肝急性期、乙肝及丙肝患儿ＰＢＭＣｓＴＮＦα、ＩＬ－６、ＩＬ－８均较正常对照组升高，而α干扰素（ＩＦＮ－α）均较正常对照组降低。甲肝恢复期ＣＤ４、ＣＤ８和ＰＨＡ刺激前ＰＢＭＣ上ｍＩＬ－２Ｒ表达率以及上述各细胞因子水平均恢复正常。提示细胞免疫功能紊乱与小儿病毒性肝炎的发病及肝损害过程有关。
To investigate the cellular immunological changes in children with viral hepatitis. The authors determined T lymphocyte subsets and the expression rate of membranous IL2 receptor (mIL2R) of lymphocytes in peripheral blood in 56 cases (Hepatitis A 17, chronic hepatitis B 28 and hepatitis C 11) by using an alkaline phosphotase antialkaline phosphatase method. The levels of interleukin 6 and 8, interferon α and tumor necrosis factor α (IL6, IL8, IFNα, TNFα) in supernatant of cuitured peripheral blood mononuclear cells (PBMCs) of 49 cases (17, 21 and 11 cases with hepatitis A, B and C respectively) were measured. The results showed that the subproportion of CD4 was lower and CD8 higher in 3 viral hepatitis groups than that in normal control. Before stimulation with PHA, the rate of expression of mIL2R in acute stage of hepatitis A was significantly higher than those of the other groups. After stimulation with PHA, the rate of expression of mIL2R significantly increased in all groups compared to the prestimulation level. However, the rate of mIL2R expression in hepatitis B and C groups was lower than that in normal control. No significant difference was found among the groups of normal control, acute and recovery stage of hepatitis A. The levels of IL6, IL8, TNFα in PBMCs of all the 3 viral hepatitis groups increased and the level of INFα decreased as compared with those of normal control. But there was no significant difference among the 3 viral hepatitis groups. It was concluded that cellular immunological disorders were related to the onset and induced damage of the viral hepatitis in children.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1997年第12期641-644,共4页
Chinese Journal of Pediatrics
