摘要
目的了解江苏省福氏4c志贺菌生化特征、对抗生素耐药性及毒力基因携带情况。方法采用国际金标准细菌鉴定(API)试剂条进行生化鉴定,用玻片凝集法进行血清分型,细菌药物敏感试验纸片扩散(K-B)法检测对抗生素的耐药性,聚合酶链反应(PCR)扩增ipaH、set1、sen和ial4种毒力基因。结果福氏4c志贺菌发酵葡萄糖、甘露醇,41.0%菌株发酵阿拉伯糖。对阿莫西林/棒酸和萘啶酸耐药率高达100.0%,氨苄西林、四环素和复方新诺明耐药率为89.7%;79.5%菌株耐受一半以上抗生素。ipaH、set1、sen、ial毒力基因携带率分别为100.0%、100.0%、92.3%和92.3%,92.3%(36/39)菌株携带全部4种毒力基因。结论福氏4c志贺菌多重耐药情况较为严重,敏感药物有庆大霉素、头孢类药物和诺氟沙星。菌株毒力基因携带率高,具有较强的致病性。
Objective The study was conducted to identify the biochemical characteristics, antibiotic resistance and virulence genes of Shigella flexneri 4c isolates in Jiangsu province.Methods Biochemical identification of the strains was performed using API 20E, serotyping using slide agglutination, antimicrobial susceptibility testing using the Kirby-Bauer method and detection of ipaH, setI, sen, ial virulence genes using PCR. Results The S. flexneri 4c isolates, fermenting glucose and mannitol with 41.0% strains fermenting arabinose, were found 100% resistant to amoxicillin/clavulanate and nalidixic acid, 89.7% resistant to ampicillin, tetracycline and sulfamethoxazole; 79.5% isolates possessed resistance to more than a half of antibiotic species. Presence of ipaH, setI, sen, ial virulence genes was identified among 100.0%, 100.0%, 92.3% and 92.3% of tested isolates, respectively, while 92.3% strains （36/39） carried all four genes. Conclusion Strong multidrug resistance of S. flexneri 4c isolates was found in the present study. Susceptibility to gentamicin, cephalosporin and norfloxacin was identified. Presence of virulence genes associated with strong pathogenicity was found in a high proportion of isolates.
出处
《疾病监测》
CAS
2008年第7期412-414,共3页
Disease Surveillance
关键词
志贺菌
福氏4c
生化特征
耐药性
毒力基因
shigella
flexneri 4e
biochemical characteristics
drug-resistance
virulence gene
