摘要
目的了解精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp,RGD)多肽表面修饰的羟基磷灰石(hydroxyap-atite,HA)修复骨缺损过程中,实验动物血液流变学和骨缺损修复区血流量的变化。方法选择20只新西兰白兔,制作15mm长的桡骨节段性骨缺损模型,根据植入不同移植材料分为实验组和对照组,实验组于动物左侧桡骨缺损区植入MSC复合RGD多肽表面修饰的HA培养制备的组织工程骨,对照组植入MSC复合HA培养制备的组织工程骨,观察各组动物术后7d、14d血液流变学和术后14d骨缺损修复区血流量的变化。结果实验组与对照组比较,血液流变学指标和骨缺损修复区血流量差异显著,实验动物全身血液黏度和红细胞聚集指数降低,骨缺损修复区的局部血流量增加。结论RGD多肽表面修饰对以HA为支架材料组织工程骨的修复有明显优化作用。
Objective To study the changes on the hemorheology and blood flow volume in the bone defects area of experimental animals repaired by HA modified by RGD peptide. Method The experimental model of 15mm radial segmental defect was produced in 20 New Zealand rabbits which were divided into experiment and control groups. Tissue engi- neered bone produced by MSC cocultured with HA modified by RGD peptide was used to repair bone defect of left limbs in experiment group,while tissue engineered bone produced by MSC cocultured with HA to repair defect in control group. The changes of hemorrheology and blood flow volume in bone defects area were observed in two groups. Results The hemorheology indexes of experiment group changed significantly as compared with those of control group,the blood viscosity and erythrocyte aggregation index reduced and blood flow volume of bone defect increased during the bone defects were repaired by tissue engineered bone produced by MSC cocultured with HA modified by RGD. Conclusion The surface modification of RGD peptide could optimize the repairing effect of tissue engineered bone produced by HA as scaffold material.
出处
《北京生物医学工程》
2008年第4期341-343,351,共4页
Beijing Biomedical Engineering
基金
全军医学科学技术"十一五"计划课题基金(06MA081)资助
关键词
组织工程骨
表面修饰
血液流变学
血流量
tissue engineering
surface modification
hemorheology
blood flow volume
