摘要
目的 观察曲古菌素A（trichostatin A，TSA）对人胰腺癌PaTu-8988细胞增殖及细胞周期的影响。方法不同浓度（0．1、0．5、2．0μmol／L）TSA处理人胰腺癌PaTu-8988细胞，并设空白对照组。采用WST-8法检测细胞存活率；流式细胞仪检测细胞周期的变化；实时定量PCR检测细胞周期相关基因p21和cyclinD1mRNA的表达。结果对照组、TSA0．1μmol／L、0．5μmol／L和2．0μmol／L组的细胞存活率分别为（100．0±4．2）％、（88．5±4．2）％、（79．7±5、0）％和（64、3±7.2）％，各TSA组均显著低于对照组（P〈0．01）。TSA0．1μmol／L、0．5μmol／L组的细胞以G1期居多，2．0μmol／L组（50．29±7．53）％细胞阻滞在G2／M期。TSA各组p21mRNA表达值分别为5、29±1．16、7．79±0．41、8．61±0．73，较对照组1．00±0．08显著升高（P〈0．01）；cyclin D1mRNA表达值分别为1．134-0、12、0．42±0．06、0．19±0．06，较对照组1．00±0．07表达降低（P〈0．05）。结论TSA通过上调p21及下调cyclinD1基因的表达，导致细胞周期阻滞。
Objective To investigate the effect of trichostatin A （TSA） on inhibition of cell proliferation and cell cycle of human pancreatic cancer cell line PaTu-8988 in vitro. Methods PaTu-8988 cells were treated with different concentration TSA （0. 1, 0. 5, 2.0 μmol/L）, and blank control group was used. The growth inhibition rates of cells were measured by WST-8 method. Cell cycle and apoptosis were detected by flow cytometry; the expressions of p21 mRNA and HDAC1 mRNA were measured by Real-Time PCR. Results Survival rates of TSA 0. 1 μmol/L, 0. 5 μmol/L and 2.0 μmol/L group was （ 88.5 ±4.2 ） %, （ 79.7± 5.0 ） % and （ 64.3 ± 7.2 ） %, respectively, which were all significantly lower than （ 100.0 ± 4.2 ） % of the blank control group （ P 〈0. 01 ）. Cells in the group of TSA 0. 1 μmol/L and 0. 5 μmol/L were hindered at G2 phase, while （50.29 ＋ 7.53）% of the cells in the group of TSA 2.0 μmol/L were hindered at G2/M phase. The expression rates of p21 mRNA in different TSA group were 5.29 ± 1.16, 7.79 ± 0. 41, 8.61± 0. 73, respectively, which were higher than that in the control group （ 1.00 ±0.08, P 〈0. 01 ） ; the expression rates of Cyclin D1 mRNA were 1.13 ± 0. 12, 0. 42 ±0. 06, 0. 19 ± 0. 06, respectively, which were higher than that in the control group （ 1.00 ± 0. 07, P 〈 0. 05 ）. Conclusions TSA may up-regulate the expression of p21 and down-regulate the expression of cyclin D1, and induce cell cycle blockade.
出处
《中华胰腺病杂志》
CAS
2008年第4期234-236,共3页
Chinese Journal of Pancreatology
