摘要
目的利用Sysmex XE-2100血细胞分析仪提示的嗜酸粒细胞增高或无嗜酸粒细胞分类结果及异常散点图信息，探索能够快速、简便地检出疟原虫的方法。方法用XE-2100血细胞分析仪进行血常规检测，对嗜酸粒细胞比值增高或无嗜酸粒细胞分类结果的标本进行显微镜检查，当镜检未发现嗜酸粒细胞异常时，继续镜检红细胞，查找疟原虫。结果在1501份仪器报警提示嗜酸粒细胞比值增高或无嗜酸粒细胞分类结果的标本中，经显微镜镜检发现有9份嗜酸粒细胞结果正常，与仪器报警不符。其中6份仪器报警提示嗜酸粒细胞增高，分类散点图中的嗜酸粒细胞与中性粒细胞间的间距变小；另外3份仪器报警提示白细胞分类散点图异常，嗜酸粒细胞与中性粒细胞间无间距，从而无分类结果。但9份标本均在红细胞中检出疟原虫的滋养体、裂殖体或配殖体。结论当Sysmex XE-2100血细胞分析仪提示嗜酸粒细胞增高或无嗜酸粒细胞分类结果，而未得到显微镜镜检证实时，则高度提示有疟原虫感染。此方法不仅能快速、简便地筛查疟原虫，而且对于防止漏检疟原虫有重要临床实用价值。
Objective To explore a quick and feasible method of screening malaria parasite by using a Sysmex XE-2100 hematology analyzer though alarm information on high eosinophil count and atypical eosinophil distributions in the WBC scattergram. Methods Sysmex XE-2100 hematology analyzer was used for complete blood cell analysis. Microscopic review was used when high eosinophil count and atypical eosinophil distributions in the WBC scattergram were found. If the review showed normal eosinophil cells, we continued to focus on red cell for searching malaria parasite. Results Among 1 501 specimens showing high eosinophil counts and atypical eosinophil distributions, nine cases with normal eosinophil cells were in disaccordance with the hematology analyzer, six of them showed high eosinophil count in the Sysmex XE-2100 hematology analyzer, whose distribution was located close to neutrophil clusters in scattergram. The other three had an abnormal WBC scattergram. There was no gap between eosinophil clusters and neutrophil clusters, which brought no classified results. But all the nine specimens had been found the trophozoite, schizont and merozoite in blood smears. Conclusions There were great possibility of the existence of the malaria parasite in specimens when hematology analysis showed high eosinophil count and atypical eosinophil distributions in the WBC scattergram in a Sysmex XE-2100 hematology analyzer, although these alarm was not comfirmed by microscopic review. This method provides not only a simple and reliable way for quick screening malaria parasite but also has a great value in preventing the undetected ratio on malaria parasite.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2008年第7期763-766,共4页
Chinese Journal of Laboratory Medicine
关键词
疟疾
嗜酸细胞
血液学试验
诊断设备
自动分析
Malaria
Eosinophils
Hematologic tests
Diagnostic equipment
Autoanalysis
