摘要
目的:建立高效液相色谱(HPLC)法测定小儿氨酚黄那敏片中对乙酰氨基酚含量。方法:采用安捷伦EclipseXDB-C18色谱柱(150mm×4.6mm,5μm),甲醇-水(80∶20)为流动相,流速为0.8ml/min,柱温为30℃,检测波长为249nm。结果:对乙酰氨基酚在0.2005～2.0048μg范围内呈良好线性关系,r=0.9999,平均回收率为99.82%,RSD为0.23%(n=6)。结论:该法稳定、简便,可用于小儿氨酚黄那敏片的质量控制。
Objective：To establish an HPLC method for the determination of Paracetamol in Xiao＇er Anfen Huang Namin Tablets. Methods： Agilent Eclipse XDB-CIs（150 mm×4.6 mm, 5um） column was used. The mobile phase consisted of methanol and water（80：20）,the flow rate was 0.8 ml/min,column temperature was 30℃ and wavelength of detecting at 249 nm. Results： The linear range of paracetamol was within 0.200 5-2.004 8ug, （r=0.999 9）, the average recovery was 99.82%, RSD=0.23%（n=6）. Conclusion： The method is simple and stable. It can be used for the control of Xiao＇er Anfen Huang Namin Tablets.
出处
《中国医药导报》
CAS
2008年第24期53-54,共2页
China Medical Herald
