摘要
目的通过观察凋亡相关基因Survivin在哈萨克族食管癌癌组织及其癌旁组织中的表达来探讨Survivin哈萨克族食管癌发生中的作用。方法采用RT-PCR方法检测Survivin基因mRNA在20例哈萨克族食管癌组织及其癌旁组织标本中的表达,选取mRNA水平差异组织标本采用Western-bolt方法检测其在蛋白水平的表达情况。结果Survivin基因mRNA在20例癌组织中有10例表达,阳性率为50%,癌旁组织中仅有4例表达,癌组织与其对应的癌旁组织相比,Survivin基因的表达量均明显高于癌旁组织。Survivin蛋白的表达在癌组织与癌旁组织中无明显差异。结论提示Sur-vivin基因的异常表达主要在转录水平,其对哈萨克族食管癌发生发展过程中的作用有待于进一步研究。
Objective To study the significance of apoptosis-related gene Survivin in Kazak′s esophageal cancer.Methods The expression of Survivin gene mRNA was detected in tissues of 20 cases with esophageal cancer and tumor-adjacent normal tissues by reverse transcription polymerase chain reaction.Then the expression of Survivin protein was detected in some samples with difference in transcriptional level by Western-blotting.Results The overall positive rate of Survivin gene mRNA expression was 50% in the tissues of 20 cases wtih esophageal cancer,and 20% in tumor-adjacent normal tissues.There was no difference in the expression of Survivin level between tumor tissue and tumor-adjacent tissue.Conclusions It is suggested that the abnormal expression of Survivin gene affects the occurrence of Kazak′s esophageal cancer mainly in transcriptional level,and it should be further studied.
出处
《地方病通报》
2008年第4期4-6,共3页
Endemic Diseases Bulletin
基金
新疆维吾尔自治区科技厅重点实验室开放课题基金(XJDX0208-2007-04)
新疆维吾尔自治区高技术研究与发展计划项目(200810103)
