摘要
目的:观察霉酚酸酯(MMF)对多囊肾囊肿衬里上皮细胞增殖和凋亡的影响,并与雷帕霉素(RAPA)比较。方法:采用原代培养的人多囊肾囊肿衬里上皮细胞,用不同浓度MMF和RAPA分别作用48 h和72 h。MTT法检测细胞增殖情况;流式细胞术检测细胞周期变化;AnnexinⅤ-FITC流式细胞术测定细胞凋亡率;透射电镜观察细胞超微结构改变。结果:MMF和RAPA均能抑制囊肿衬里上皮细胞增殖,呈明显的量效和时效关系。作用48 h后MMF使细胞阻滞在S期,RAPA使细胞阻滞在G0/G1期。MMF和RAPA均可诱导细胞凋亡,最大凋亡率分别为(5.53±0.27)%和(4.36±0.10)%;电镜下可见典型的凋亡改变。结论:MMF与RAPA相比同样能明显抑制囊肿衬里上皮细胞增殖,诱导细胞凋亡,但其对细胞增殖的抑制作用较RAPA弱。
Objective：To investigate the effect of mycophenolate mofetil（MMF）on proliferation and apoptosis of cyst-lining epithelial cells in patients with autosomal dominant polycystic kidney disease （ADPKD）, and to compare its effect with that of rapamycin （RAPA）in vitro. Methods： Primary cultured cyst-lining epithelial cells were treated with MMF and RAPA at different concentrations（0,0. 005,0.05,0.5,5 μg/ml）for 48 h or 72 h. The inhibitory effects of them on the cells were evaluated by MTT assay; the cell cycle distribution and apoptotic ratio were determined by flow cytometry. The morphological changes of cyst-lining epithelial cells were observed under transmission electron microscope. Results： Both MMF and RAPA significantly inhibited the proliferation of cyst-lining epithelial cells in a dose- and time-dependent manner. After 48 h treatment,the cells were blocked at S phase by MMF and at G0/G1 phase by RAPA. Both drugs induced cell apoptosis,with the maximal apoptotic rate being （5. 53 ± 0. 27）% for MMF and （4. 36 ± 0. 10）% for PAPA. Typical morphological changes of apoptotic cells were observed under electron microscope. Conclusion： MMF can effectively inhibit proliferation and induce apoptosis of cyst-lining epithelial cells, but its inhibitory effect is weaker than that of RAPA.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2008年第8期921-924,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30570867,30330640,30271523)~~
关键词
霉酚酸酯
雷帕霉素
多囊肾
囊肿衬里上皮细胞
细胞增殖
细胞凋亡
mycophenolate mofetil
rapamycin
polycystic kidney disease
cyst-lining epithelial cell
cell proliferation
apoptosis
