摘要
目的:探讨中药地黄对大鼠肾纤维化的作用及可能的机制。方法:将实验动物分成正常对照组、模型组、地黄组,采用一次性尾静脉注射阿霉素复制大鼠肾病模型。治疗8周后观察尿蛋白、尿素氮、血肌酐及肾脏病理改变;同时观测肾皮质中转化生长因子β受体Ⅰ(TβRⅠ)、转化生长因子β受体Ⅱ(TβRⅡ)和Smad2、7的表达。结果:地黄组各项指标与模型组相比,均有统计学差异,形态学观察也显示其组损害轻于模型组。且模型组肾组织TβRⅠ、Smad2 mRNA显著上调,地黄组肾组织TβRⅠ、Smad2 mRNA显著下调,而模型组肾组织Smad7蛋白表达显著下调。同时TβRⅡ mRNA的表达无明显变化。结论:在阿霉素肾病大鼠模型中,地黄提取物对大鼠肾脏有一定的保护作用,其信号转导机制至少部分与它能下调TβRⅠ、Smad2及上调Smad7的表达有关。
Objective：To explore the effect and mechanism of Dihuang in treating adriamycin （ADR） induced renal fibrosis rats. Methods： The Nephropthy model rats established by a single intravenous injection of Adriamycin were divided into the normal group, the model control group and the Dihuang group. 24 h urinary protein excretion, albumin, serum creatinine, BUN and pathological cbanges of kidney were examined and the expression levels of transforming growth factor β receptor Ⅰ（TI3R Ⅰ ）, transforming growth factor β receptor Ⅱ （TβR Ⅱ ）, Srnad 2 and Srnad 7 were detemined after 8 weeks treatment. Results： After treatment, all the parameters between the Dihuang group and model group were significantly different respectively;pathological changes in the Dihuang group were milder than those in the model group. The mRNA expressions of TβR Ⅰ and Smad 2 in kidney tissue of the model group were significandy upregulated and those of TβR Ⅰ and Smad 2 in kidney tissue of the Dihuang group were significantly downregulated and the protein expression of Smad 7 in kidney tissue of the model group were significantly downregulated, while the mRNA expression of TβR Ⅱ remained unchanged. Conclusion： Dihuang significantly prevents against renal injury in the rats with nephropathy partially by downregulating the expression of TβR Ⅰ and Smad 2 and upregulating the expression of Smad 7.
出处
《中国中西医结合肾病杂志》
2008年第8期684-686,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
浙江省教育厅基金资助项目(No.20051087)
