摘要
采用DAPI-荧光染色法研究松材线虫、拟松材线虫、李氏长尾线虫和吴氏长尾线虫等两对形态相似的姊妹线虫的成虫生殖系细胞学特征,描述并且比较了两对姊妹线虫之间以及姊妹线虫对之间的成虫生殖系细胞排列方式和贯穿于生殖细胞分裂过程中的染色体变化行为,同时也图示了这4种线虫的成虫生殖系细胞核形态图和染色体形态图。研究表明,利用线虫成虫生殖系细胞学特征来探讨线虫种类之间甚至个体之间的相互关系,将可能便于人们更好地界定线虫"种"的范围及解释线虫进化和系统发育过程;研究还表明,经DAPI-荧光染色的生殖系细胞排列模式可能可以作为一种较好的线虫分类特征。此外,本研究的4种线虫的染色体数目均为2n=12。
With DAPI staining, structures of the young adult germ lines from two pairs of morphologically cryptic species of Bursaphelenchua xylophilus and B. mucuonatus and Seinura and S. wuae were characterized. Within and between the two pairs of sibling species, germ-cell arrangement patterns and chromosome behaviour included in germ cells divisions were described and compared. Nuclear morphologies of the four young adult germ-lines were illustrated. This study suggested that cytological features from the germ-line of nematodes might assist taxonomic and systematic researchers to better understand the relationships between and within nematode species. It also indicated that the germ-cell arrangement patterns revealed by DAPI-fluorescence method might be a sound taxonomic character for use in nematode classification. In addition, six bivalents of chromosomes were observed in both male and female germ cells in all four species studied.
出处
《动物分类学报》
CSCD
北大核心
2008年第3期435-442,共8页
Acta Zootaxonomica Sinica
基金
This project was supported by the National Natural Science Foundation of China(30471392)
