摘要
目的:探讨人肿瘤坏死因子α(TNF-α)联合紫杉醇对人腺样囊性癌细胞系SACC-83的抑制增殖作用及与细胞凋亡的关系,为化疗与生物治疗联合应用治疗腺样囊性癌提供实验依据。方法:将人腺样囊性癌细胞系SACC-83和对照组原代培养鼠肺成纤维细胞根据不同药物浓度分为9组,药物作用72 h。3H-TDR法检测细胞增殖抑制率;流式细胞术检测细胞凋亡率及细胞周期改变;透射电镜观察凋亡细胞形态变化。结果:联合用药组的细胞增殖抑制率显著高于单独用药组(P<0.05)。L929细胞各组的细胞增殖抑制率显著低于SACC-83细胞(P<0.05)。结论:TNF-α联合紫杉醇对SACC-83细胞增殖的抑制作用以及诱导凋亡具有协同作用。
Objective： To explore the apoptosis mechanism of salivary adenoid cystic carcinoma cells （SACC-83） induced by recombinant human tumor necrosis factor or（rhTNF-α） and paclitaxel. Methods： The SACC-83 cells and primary culture of L929 cells were divided into nine groups of different medicine concentration and exposed for 72h. The inhibition ratio of cell proliferation was detected with 3H-TDR. The percentage of apoptotic cells and cell cycle distribution were detected by the flow cytometer. The cell morphological change of apoptosis was observed. Results： The inhibition ratio of cell proliferation in as- sociated drugs group was significant higher than that of either single drug group （ P 〈 0.05 ）. The inhibition ratio of cell prolifera- tion of every group of L929 cell was significant lower than that of SACC-83 cell （P 〈 0.05）. Conclusion： TNF-αassociated with paclitaxel is synergetic to inhibit SACC-83 cells and induce apoptosis.
出处
《军医进修学院学报》
CAS
北大核心
2008年第4期265-267,共3页
Academic Journal of Pla Postgraduate Medical School
