摘要
目的研究大黄素与顺铂(DDP)联合应用对人肝癌细胞株HepG2生长抑制与促凋亡作用。方法大黄素与顺铂联合应用肝癌细胞株HepG2分别24h、48h、72h后,MTT法检测细胞生长抑制率,流式细胞术检测凋亡,并与单药大黄素、单药顺铂作用相比较,计算两药相互作用系数,检验其协同性。结果大黄素和顺铂单独应用48、72hHepG2细胞抑制率分别为21%、28%和29%、34%,二药联合时抑制率为49%和61%,3个药物组CDI值分别为0.93、0.91、0.82,流式细胞术显示二药联用较单药有更明显的促凋亡作用。结论大黄素单药应用能抑制HepG2细胞生长增殖,诱导凋亡,与顺铂联用能增强其抗肿瘤作用,二药有协同效应。
Objective To explore the suppressing effect of proliferation and apoptosis induction on HepG2 cells by treating with emodin combining with cisplatin, and evaluate the coefficient effect of two drugs. Methods Treating HepG2 cells with emodin and cisplatlon for 24,48,72h, then the inhibition rate was evaluated by MTT assay and apotosis rate was detected by flow cytometer. The result was compared with using those drugs alone. Results When treated with emodin or cisplatin for 48h and 72h, the inhibition rate is 21%, 28% and 29% ,34% respectively. After treated with these two drugs together,inhibition rate is elevated to 49% in 48h and 61% in 72h. The CDI in 24h ,48h and 72h is 0.93,0.91, and 0.82 respectively. The apoptosis rate in drug combination group is higher than it in emodin or cisplatin alone group. Conclusion Emodin can supress the proliferation and induce apoptosis of HepG2 cells. When combinede with cisplatin, this effect was enhanced. These two drugs have coefficient effect.
出处
《四川医学》
CAS
2008年第8期963-964,共2页
Sichuan Medical Journal
