摘要
目的:建立柱切换高效液相色谱(HPLC)法直接进样测定人血浆中替米沙坦浓度。方法:血浆样品直接进样,经限进填料(RAM)预柱用甲醇-水(10:90)在线净化和富集,然后洗脱切入 C_(18)分析柱用甲醇-水(63:37)进行分离,荧光检测λ_(ex)302nm/λ_(em)365nm。结果:标准曲线线性范围10～2000μg·L^(-1)(r=0.9995);回收率为95.4%～97.7%(n=5);日内 RSD 为0.2%～2.6%(n=5),日间 RSD 为2.1%～4.1%(n=5);最低检测浓度为0.51μg·L^(-1)。结论:本法简便、可靠,适用于测定人血浆中替米沙坦浓度及其药动学的研究。
Objective：To establish a column -switching high performance liquid chromatographic （HPLC） method for the determination of telmisartan in human plasma by direct injection. Methods：The plasma samples injected directly onto the pre -column packing with restricted -access materials（RAM） were on -line washed with methanol -water（ 10： 90）,and the component were eluted onto analytical column to separate with methanol- water（63： 37）. The flow rate was 1.0 mL · min^-1 with fluorescence detection set at λex 302 nm and λem 365 nm. Results：The calibration linear range for telmisartan in plasma was 10 - 2000 μg · L^- 1 with correlation coefficient of 0. 9995 while the limit of quantification was 0.51 μg · L^-1. The recoveries of method were 95.4% -97.7% （ n =5 ）. The RSD of intra - day and inter - day were 0.2% -2.6% and 2.1% -4. 1% （ n =5 ） ,respectively. Conclnsions：This method is simple and reliable for the determination of telmisartan in human plasma and its pharmacokinetic study.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第8期1071-1073,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
替米沙坦
高效液相色谱
限进填料
柱切换
telmisartan
HPLC
restricted - access media
column - switching
