摘要
采用初始能量为100MeV/u的重离子束12C6+对紫花苜蓿下胚轴及子叶外植体进行辐照处理,研究重离子辐照对愈伤组织诱导状态及诱导率、愈伤组织相对生长率、体细胞胚诱导率及植株再生的影响。结果表明,重离子辐照对下胚轴及子叶愈伤组织的诱导具有抑制作用,且出愈率随着辐照剂量的增大而降低;在继代培养过程中,其愈伤组织相对生长率均高于对照组,外植体本身对重离子辐照所造成的损伤具有恢复能力;辐照处理对体细胞胚胎诱导也有影响,30Gy时,下胚轴诱导的体细胞胚胎发生较对照组早,数量多,较早地得到再生植株;而50Gy时,所得到的体细胞胚未能发育成再生植株。同时应用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对重离子辐照处理下胚轴所得再生植株进行检测分析,结果表明:所采用的20条随机引物中有11条在对照及处理组所得再生植株之间扩增出差异性多态条带,表明了重离子辐照引起苜蓿再生植株基因组DNA发生变异。
The hypocotyls and cotyledons of Medicago sativa were irradiated with original energy 100 MeV/u heavy ion beam ^12C^6＋. The effects of irradiation on the growth state and the callus induction rate, the callus relative growth rate, the somatic embryogenesis rate and plantlet regeneration were studied. The results show that heavy ion beam irradiation can inhibit the callus induction of the hypocotyls and cotyledons, and the frequency of callus declines gradually with the increment of irradiation doses. In the course of subculture, their callus relative growth rates are higher than that of the controls, and the hurt caused by irradiation has been restored. The heavy ion beam irradiation has an influence on the somatic embryogenesis rate. The somatic embryo induced by the hypocotyls irradiated up to 30 Gy formed earlier and the number is larger while irradiated at 50 Gy there is no plant obtained. The results of RAPD （Random amplified polymorphic DNA） analysis on the control and the plants induced by the hypocotyls irradiated within 10 - 40 Gy suggest that eleven among twenty random primers have been amplified with different DNA polymorphism bands. Consequently, heavy ion beam irradiation could cause mutation of alfalfa genome DNA.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2008年第4期228-232,共5页
Journal of Radiation Research and Radiation Processing
基金
中国科学院西部之光基金(XL050616)资助
