摘要
[目的]优化安祖花愈伤组织增殖与分化的培养基。[方法]以安祖花愈伤组织为材料,研究基本培养基、外源激素6-BA和NAA对其增殖、分化及分化苗质量的影响,同时检测内源激素含量的变化,观察分化苗的表型特征。[结果]安祖花愈伤组织增殖的适宜基本培养基为：改良MS（1/2NH4NO3＋1/2CaCl2）,增殖倍数达4.97倍。6-BA和NAA均影响安祖花愈伤组织的增殖、分化,在适宜的温度条件下,当6-BA浓度为0.75mg/L时,愈伤组织增殖较快,分化苗的质量好。安祖花愈伤组织增殖与分化适宜的NAA浓度范围为0.05～0.2mg/L。外源激素的变化引起了安祖花愈伤组织内源激素的改变。[结论]安祖花愈伤增殖与分化的适宜培养基为：MS（1/2NH4NO3＋1/2CaCl2）＋0.75mg/L6-BA＋0.1mg/LNAA＋30g/L白糖＋7.0g/L琼脂粉（pH值5.8～6.0）。
[Objective] The aim was to optimize the medium for proliferation and differentiation of Anthurium callus. [Method] With Anthurium callus as material, the effect of basic medium, exogenous hormone, 6-BA and NAA on the quality of proliferation and differentiation and differentiation seedlings of Anthurium callus was studied, the content change of endogenous hormones was detected, and phenotypic features of differentiation seedlings were observed. [Result] The suitable basic medium of proliferation of Anthurium callus was： the improved MS（1/2 NH4NO3＋1/2 CaCl2）, proliferation multiple was up to 4.97 times. Both of 6-BA and NAA impacted the proliferation and differentiation of Anthurium callus, when the concentration of 6-BA was 0.75 mg/L, the proliferation of callus was fast, and quality of differentiation seedlings was good under the appropriate temperature condition. The proliferation of Anthurium callus and NAA concentration range of suitable differentiation was 0.05-0.2 mg/L. The change of endogenous hormones was arisen by variation of exogenous hormone of Anthurium callus. [Conclusion] The suitable basic medium of proliferation and differentiation of Anthurium callus was MS（1/2 NH4NO3＋1/2 CaCl2）＋0.75 mg/L 6-BA＋ 0.1 mg/ L NAA＋30 g/L sugar＋7.0 g/L agar powder（pH 5.8-6.0）.
出处
《安徽农业科学》
CAS
北大核心
2008年第21期8925-8927,共3页
Journal of Anhui Agricultural Sciences
