摘要
为提高白花泡桐繁殖效率,防止丛枝病的传播,保持优良种性,利用愈伤组织再生技术对其增殖.研究表明:叶柄产生愈伤组织最适培养基为MS+2.0 mg/L BA+0.2 mg/L NAA+0.05 mg/L2,4-D;诱导芽分化最适培养基为1/2MS+2 000 mg/L脯氨酸,可使诱导分化率达27%;增殖继代最适培养基为B5+1.0 mg/L BA+0.1 mg/L NAA,增殖系数达6.79;生根最适培养基为1/2MS+0.1 mg/L IBA+0.1 mg/L NAA,生根率达100%.
For developing propagation, preventing the spreading of Paulownia Witches＇ - broom （PaWB） and holding excellent variety, the technique of plant regeneration from petiole calli of Paulownia fortunei and rapid propagation was studied. The result showed that MS ＋ BA2.0 mg/L ＋ NAA0.2 mg/L ＋ 2.4-DO. 05 mg/L was the best culture medium of callus induction. The suitable medium for regeneration was 1/2MS ＋ 2 000 mg/L proline. The regeneration frequency can be 27%. The best culture medium of propagation was B5 ＋ BA1.0 mg/L ＋ NAA0.1 mg/L. The propagation quotient was 6.79.1/2MS ＋ IBA0. 1mg/L ＋ NAA0. 1mg/L was the best culture medium of inducing the rhizogenesis . The rate of rhizogenesis was 100%.
出处
《信阳师范学院学报（自然科学版）》
CAS
北大核心
2006年第4期407-410,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
河南省科技攻关项目(0524050005)
关键词
白花泡桐
愈伤组织
再生植株
Paulownia fortunei
callus
plant regeneration
