摘要
目的:观察瘦素对培养大鼠原代肝细胞葡萄糖吸收及肝细胞膜胰岛素受体磷酸化的影响,探讨瘦素参与胰岛素抵抗的分子生物学机制。方法:体外培养大鼠肝细胞,根据培养基中加入瘦素的浓度将实验分为空白对照组以及50、100、200、500μg/L瘦素组,共5组,每组分别培养2h和24h,用全自动生化分析仪检测培养基中剩余的葡萄糖浓度,用ELISA方法测定肝细胞膜磷酸化胰岛素受体β亚基(pY1158)含量。结果:①培养基剩余葡萄糖检测,瘦素抑制肝细胞对葡萄糖的吸收,且抑制效应随瘦素浓度增加和时间延长而增强(P<0.01);②肝细胞膜磷酸化胰岛素受体β亚基(pY1158)水平测定,50μg/L瘦素组与对照组比较差异无统计学意义(P>0.05),100、200、500μg/L瘦素组与对照组比较差异有极显著性(P<0.01),并随瘦素剂量上升含量进一步下降。结论:瘦素与胰岛素抵抗相关,抑制细胞膜胰岛素受体β亚基(pY1158)磷酸化可能是其参与胰岛素抵抗的机制之一。
Objective：To observe the effects of leptin on glucose absorption and insulin receptor phosphorylation in primary cultured rat hepatic cells, and explore the molecular biological mechanism of leptin in insulin resistance. Methods： Primary cultured rat hepatic cell was divided into 5 groups according to the different doses of leptin （blank, 50/μg/L, 100/μg/L, 200/μg/L and 500/μg/L） incubated. Each group was incubated for 2 hours and 24 hours respectively. Residue glucose concerntration was determined by automatic biochemistry analyzer and content of insu- lin receptor phosphorylation pYl158 was deteceted by ELISA kit. Results： ①Leptin can inhibit glucose absorption in hepatic cell, and the effect was dose-dependent and time dependent（P〈0.01）. ②There was no significant difference in level of insulin receptor phosphorylation between leptin 50/μg/L group and control group（P〉0.05）, however, there were significant differences between all the other leptin dose groups and control group（P〈0.01）, and these effects were dose-dependent. Conclusion： Leptin is correlated with insulin resistance, and it＇s inhibitory effect on insulin receptor phosphorylation pYll58 may be one of the mechanisms in insulin resistance.
出处
《内科急危重症杂志》
2008年第4期178-180,193,共4页
Journal of Critical Care In Internal Medicine
关键词
瘦素
糖代谢
胰岛素受体磷酸化
肝细胞
胰岛素抵抗
Leptin Glucose metabolism
Insulin receptor phosphorylation
Hepatic cell
Insulin resistance
