摘要
应用免疫细胞化学PAP法系统研究了大鼠胚胎13天至成年食道及胃神经激肽A发生发育过程.结果如下:(1)在食道,直至出生前,即胚胎21天才于环肌层及粘膜肌层出现阳性膨体纤维,出生后,随幼鼠的生长发育,相继在上皮内、纵肌层、肌间丛、粘膜下丛出现神经激肽A免疫反应阳性物,其纤维的密度、粗细和着色也逐渐增加,30天时已具备成年的分布特征,其发育主要在生后4周;(2)在胃,于胚胎14天,首先在肌间丛处呈现阳性反应,且发育迅速,阳性反应强,相继在环肌、纵肌、粘膜下层血管壁出现阳性纤维,出生后又于粘膜肌、固有膜及上皮内出现阳性纤维,30天时已和成年鼠的相似,其发育主要在生前1周至生后4周内.提示同一种神经肽在消化道的不同节段其发生不同步,且具不同的发育规律,反映了神经激肽A与消化道的活动有密切的关系.
The occurrence and development of neurokinin (NKA) positive nerves in rat esophagus and stomach were observed from 13th day of embryo to adult with the method of PAP immunocy-tochemistry. The results were as follows; (1) In rat esophagus, the positive varicose fibres could be found in the circular muscle layer and the muscularis mucosae layer at 21 days before birth. After birth, NKA-immunoreactivity positive nerves could be found in the epithelium, the longitudinal muscle layer, the myenteric plexus and the submucosal plexus. With increasing age, the density, thickness, and stain of NKA-I positive nerves increased gradually. Mature nerve fibres could be seen at one month. (2) In rat stomach, the positive reaction of the nerves initially happened in the myenteric plexus, and then the positive fibres appeared in circular muscles, longitudinal muscles and submacosa blood vessels. 30 days after birth, they became well developed. The devolopment of NKA-I positive nerves happened before birth to 4 weeks after birth. In brief, the neruopeptide developed in different positions and in different ways, which shows that NKA has a close relationship with the action of the digestive tract.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1997年第5期422-425,共4页
Chinese Journal of Anatomy
