摘要
目的:研究慢性吸烟对哮喘豚鼠肺组织中神经激肽A(NKA)含量及NKAmRNA表达的影响,探讨慢性吸烟对哮喘肺组织中NKA含量影响的机制。方法:用卵蛋白超声雾化法复制豚鼠哮喘模型;随机分成:①诱喘前吸烟2周组,②诱喘后吸烟2周组,③诱喘后2周对照组(非吸烟),④常规诱喘组(非吸烟),⑤正常对照组。用酶联免疫法检测各组肺组织中NKA含量;用RT-PCR方法检测各组肺组织中NKAmRNA的相对含量。结果:①哮喘豚鼠肺组织中NKA含量及NKAmRNA表达均明显高于正常豚鼠(均P<0.01);②诱喘前吸烟2周豚鼠,诱喘后其肺组织中NKA含量及NKAmRNA表达均明显高于非吸烟诱喘豚鼠(均P<0.01);③诱喘后吸烟2周豚鼠,其肺组织中NKA含量及NKAmRNA表达均显著高于诱喘后2周对照豚鼠(均P<0.01)。结论:结果提示:①NKA可能参与了卵蛋白诱发的豚鼠哮喘发病机制;②慢性吸烟刺激可能增加哮喘豚鼠肺组织中NKA含量;③慢性吸烟刺激可能具有在转录水平上调NKAmRNA表达的作用而导致肺内NKA蛋白含量增多。
AIM： To study the changes of neurokinin A （NKA） and NKA mRNA in lung tissues of asthmatic guinea pigs exposed to chronic cigarette smoke and investigate the mechanism. METHODS： The model of asfiuna in guinea pigs was made by exposure to aerosolized ovalbumin and animals were randomly assigned into： ① inhale cigarette smoke （ICS） 2 weeks before provoked; ② ICS 2 weeks after provoked; ③2 weeks after provoked （no - ICS） ; ④ asthma （no- ICS before provoked） ; ⑤ normal control. The contents of NKA in lung tissues were detected by ELISA and NKA mRNA expression was detected by reverse transcription - polymerase chain reaction. RESULTS： ① The levels of NKA and NKA mRNA expression in lung tissues of asthma group were significantly higher than those in normal control group （ P 〈 0.01, respectively） ; ② The levels of NKA and NKA mRNA ex- pre＆sion in lung tissues of ICS 2 weeks before provoked were significantly higher than those in asthma group （ P 〈 0.01, respectively） ; ③ The levels of NKA and NKA mRNA expression in lung tissues of ICS 2 weeks after provoked were significantly higher than those in no- ICS after provoked （P 〈 0.01, respectively）.CONCLUSIONS： ① NKA probably takes part in the pathogenesis of asthma in guinea pigs induced by ovalbumin. ② Chronic cigarette smoke may increase the levels of NKA in lung tissue of asthmatic guinea pigs. ③ Chronic cigarette smoke may up - regulate the NKA mRNA expression in lung of asthma and then elevate NKA contents.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第9期1720-1723,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.39900161)
关键词
吸烟
哮喘
豚鼠
神经激肽A
Smoking
Asthma
Guinea pigs
Neurokinin A
