摘要
本文用脂质体法将人Ｂ７－１基因导入小鼠前胃癌细系ＦＣ及人白血病细胞系ＨＬ６０，获得了稳定高效表达Ｂ７－１分子的ＭＦＣ和ＨＬ６０克隆（分别命名为Ｂ７和ＨＬ６０－Ｂ７）。在ＭＦＣ－Ｂ７及ＨＬ６０－Ｂ７刺激鼠脾淋巴细胞的增殖实验中，野生型肿瘤细胞中只能引起微弱的增殖反应，而转染Ｂ７－１分子的肿瘤细胞能非常显著刺激脾细胞的增殖（Ｐ＜０．００１）。这种效应能被抗Ｂ７－１单抗所阻断。结果表明人Ｂ７－１分子能为鼠淋巴细胞的活化增殖提供辅助刺激信号。
e transfected human B71(CD80) gene to mouse gastric carcinoma cell line MFC and human leukemia cell line HL60 with lipofectamin. The tumor cell clones of high expression B71 molecule were screened by immunohistochemstry method (named MFCB7 and HL60B7 respectively).Compared with MFC and HL60 cells ,MFCB7 and HL60B7 could significantly stimulate proliferation of mouse spleen cells respectively(P<0.001).The stimulate effects could be inhibited by antiB71[KG*4〗mAb(BB1). The results suggest that human B71 molecule can costimulate proliferation of mouse spleen cells.
出处
《基础医学与临床》
CSCD
1997年第6期55-58,共4页
Basic and Clinical Medicine
基金
国家教委细胞增殖与调控生物学开放实验室开放课题
