摘要
目的分析我院血培养标本各种病原菌分布情况及主要病原菌的耐药特点。方法不同患者选择合适的标准需氧瓶、厌氧瓶,中和抗生素培养瓶及儿童专用培养瓶,采用BACT/ALERT3D全自动血培养仪对1887份血液标本进行血培养,分离所得菌株用MICROSCANPc12/NC21鉴定药敏系统进行鉴定和药敏试验。结果培养时间缩短至5天,血培养的阳性率为8.2%(155/1887),155株阳性病原菌中革兰阳性球菌占43.2%(67/155),革兰阴性杆菌占48.4%(75/155),真菌占7.1%(11/155),革兰阳性杆菌占1.3%(2/155)。最常见的病原菌是大肠埃希菌和金黄色葡萄球菌,未发现耐万古霉素的革兰阳性球菌,革兰阴性杆菌对碳青霉烯类抗菌素高度敏感。结论全自动血培养仪提高了培养阳性率,缩短了培养时间,为血行性感染的早期诊断及合理用药提供实验依据。
Objective To investigate the distribution and drug resistance of pathogens in blood cultures of clinical samples. Methods A total of 1887 samples of blood fluid were cultured by BACT/ALERT 3D automated blood culture system with standard aerobic vials, anaerobic vials, antibiotic-neutralized culture vials and peds plus vials performed for different patients, The isolated bacteria were identified by using the MICROSC AN Pc 12/NC21 system. Results Less than 5 days were expended for culture by the method of BACT/ALERT 3D.Of all the cultures, 155 strains of pathogens were isolated at a positive rate 8.2% （155/1887） ,of the 155 strains,43.2% were gram positive coccus （155/1887）, 48.4% were gram negative bacilli （75/155）, 1.3% were gram positive bacilli （2/155） and 7.1% were fungi （11/155）. Data also showed that Escheriehia eoli and Staphylococcus aureus were dominant species whereas neither vaneomyein-resistant gram positive coccus nor earbapenera-resistant gram negative bacilli were found. Condusion The positive detection rate has been raised and the detection time reduced by BACT/ALERT 3D automated blood culture system, this result provided the evidence for early diagnosis and drug treatment against bloodstream infection.
出处
《海南医学》
CAS
2008年第9期36-38,共3页
Hainan Medical Journal
关键词
血培养
病原菌
药敏
Blood culture
Pathogen
Drug sensitivity
