摘要
以岭南黄鸡为实验动物,利用RT-HPLC法为定量手段,研究中药复方连翘制剂以20 g/kg(其中黄芩苷和绿原酸含量分别为158和53 mg/kg)单剂量经胃灌服给药后在鸡体内的药物代谢动力学特征,用3p97药动学软件的非房室模型统计矩原理分析药物动力学参数,有效药物成分绿原酸、黄芩苷在鸡体内的主要药动学参数如下:AUC分别为(10.59±0.69)和(11.15±0.78)(mg/L).h,AUMC分别是(106.06±3.11)和(138.44±2.86)(mg/L).h2,MRT分别是(10.01±0.68)和(12.42±0.43)h,t1/2分别为(6.93±0.18)和(8.61±0.36)h,Cmax分别为(0.98±0.25)和(1.04±0.25)mg/L,tmax分别为(1.93±0.15)和(2.45±0.19)h。结果表明2种有效成分在鸡体内血药浓度的变化表征了中药复方连翘在鸡体内代谢的变化规律,血药浓度-时间关系曲线均出现双峰现象,表示可能存在肝肠循环或胃肠循环,本研究为该复方制剂的临床应用提供了参考依据。
Pharmacokinetics of Chlorogenic acid and Baicalin, two active components of the compound traditional chinese medicine Weeping Forsythia were studied in chickens after oral administration of a single dose （20 g/kg）. The contents of chlorogenic acid and baicalin were 53 and 158 mg/kg in the compound. Drug concentrations in plasma were determined using high performance liquid chromatography （HPLC） assay. The pharmacokinetic parameters were calculated by 3p97 program. The pharmacokinetic process of Chlorogenic acid and Baicalin were all fitted with no-compartment model. The main pharmacokinetic parameters of chlorogenic acid and baicalin in plasma were as follows： Area under the concentration-time curve （AUC）, aera under the moment curve（AUMC）, mean residence time （MRT）, elimination half-life（t1/2 ）, maximum drug concentration（Cmax） , time of reaching maximum drug concentration （tmax） in plasma were （10. 59 ± 0. 69） and （11.15±0.78） （mg/L）· h,（106.06±3.11） and （138.44±2.86）（mg/L） · h^2,（10.01± 0. 68） and （12.42±0.43）h,（6.93±0. 18） and （8.61±0. 36）h, （0.98±0.25） and （1.04±0.25） mg/L, （1.93±0. 15） and （2.45±0.19） h, respectively. The concentration change of active constituents components in plasma can reflect the metabolism rule of compound in chicken. The double peak of plasma concentration meant that there might be hepatoenteral circulation or stom- ach intestine circulation during the metabolism process. This study could provide references for the medicine in clinical application.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第8期1116-1121,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省科技攻关项目(2004A20901003)
关键词
复方连翘
绿原酸
黄芩苷
鸡
药动学
compound Chinese traditional medicine Weeping Forsythia
chlorogenic acid
baicalin
chicken
pharmacokinetics
