摘要
10只正常成年 S-D 大鼠,静脉注射实验性大剂量 DPH(45mg/kg)后,15和180min 取中脑下丘神经组织和静脉血进行血清药物浓度检测和形态学观察。电镜观察发现给药15min 时突触前活性区内的突触小泡明显增多,血清 DPH 浓度为78.11±10.03μ8/ml;给药180min 时突触小泡较15min 为少,但较正常大鼠多,伴有突触后区肿胀和线粒体轻度变化,此时血清 DPH 浓度为20.98±4.12μg/ml。经计量分析,正常组、15min 组和180min 组 N_A 有高度显著性差异。结果提示 DPH 作用于突触前活性区,提高突触前膜稳定性,阻抑兴奋性介质的释放。
Each of 10 normal adult female S-D rats was injected with highdose DPH(45mg/kg)through a vein. Venous blood was taken for DPH levels and the nvevous tissue of colliculus posterior was also tak- en for ultrastructural examination 15 and 180 min after the infusion. Control rats were given nothing At 15min. the average DPH serum concentration is 78. 11μg/mg, While at 180 min, 20. 98μg/mg. Under the electromicroscope the synaptic vesicles in synapsis anterior increased 15 min after infusion. At 180 min of postinfusion, the synaptic vesicles were few than those at 15min, but more than those of normal rats. The slightly degeneated mitochondria were also fund at 180 min of postinfusion. The quantity of synaptic vesicles N_A(Number of vesicles/Area of synapsis anterior)was analysed by ASK 68K Graphic Analyzed and statically singnificant. The above results indicate that high-dose DPH af- fects selectively brainstem of rats in ultrastructure,but the changes were reversible from the observa- tion. The data reveal that DPH may enhance the stability of synaptic membranes and inhibit the re- lease of excitive transmitter insynapsis.
关键词
苯妥英钠
神经突触
药效
phenytoin/PD
synapsis/DE
synaptic vesieles/CY
