摘要
目的分析胆固醇酯转运蛋白(CETP)血浆水平及其TaqⅠB基因多态性与2型糖尿病(T2DM)的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测103例健康对照组、102例T2DM患者CETP第一内含子TaqⅠB多态性基因型,ELISA方法检测血浆CETP浓度,探讨了其对血脂、脂蛋白和apo水平的影响。结果T2DM组血浆CETP水平明显高于对照组;两组CETPTaqⅠB多态性基因型和等位基因频率分布差异无显著性意义,与性别、家族史、吸烟史及体质指数(BMI)无明显相关性。对照组CETPTaqⅠB等位基因型与血脂水平之间无明显关联性,但T2DM组不同基因型间HDL-C与apoAⅠ水平差异有统计学意义,B1等位基因频率与低HDL-C血症密切相关。结论CETP水平、CETPTaqⅠB基因多态性与T2DM脂代谢存在一定的相关性,可能是糖尿病脂代谢异常的重要遗传因素。
Objective To explore the relationship between plasma cholesterol ester transfer protein （CETP） and TaqlB gene polymor- phism and type 2 diabetes mellitus （DM）. Methods The genotypes of the first intron of CETP TaqlB gene in 102 patients with type 2 DM and 103 healthy controls were analyzed by polymerase chain reaction-restricted fragment length polymorphism （PCR-RFLP） , and plasma CETP were measured by enzyme-linked immunosorbent assay （ELISA）. The association of gene polymorphism with plasma lip- ids, lipoproteins and apolipoproteins was also analyzed. Results No significant differences in both of the alleles frequencies and geno- types in type 2 DM were noted in comparison with controls. There were no relationships between CETP TaqlB gene polymorphism and gender, family history, smoking and body mass index （BMI）. No significant differences of plasma lipid levels were observed in healthy controls with different genotypes. However, significant differences in level of HDL-C or apoAI among different CETP TaqlB genotype groups were observed, and significant correlation between low high-density lipoproteinemia and B1 allele in type 2 DM was observed. Conclusion CETP TaqlB gene polymorphisms associated with lipid metabolism, which may be an important genetic factor for abnormal lipid metabolism in type 2 DM.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2008年第4期297-300,共4页
Chinese Journal of Clinical Laboratory Science
关键词
2型糖尿病
胆固醇酯转运蛋白
基因型
等位基因
type 2 diabetes mellitus
cholesterol ester transfer protein
genotype
allele
