摘要
以猪传染性胃肠炎（ＴＧＥ）弱毒疫苗经与胃肠道疾病相关的足阳明胃经６个穴位和督脉经后海穴位共７个穴位的穴位免疫筛选试验，证明后海穴接种为３／３保护，是首选的接种穴位。以后海穴取代ＴＧＥ苗原鼻内途径的第二次接种，免疫４头妊娠母猪，对所产仔猪的保护数为３７／４１。以小剂量０．１ｍｌ及０．２ｍｌ后海穴接种３日龄仔猪，保护率分别为６０．５３％及１００％，而口服０．１ｍｌ只保护６．６７％。针刺１．５ｃｍ及３．０ｃｍ深度注苗的穴位刺激参数试验均获全保护。针刺点旁离穴位０．５ｃｍ及１．０ｃｍ分别获１００％及７５％保护，而同时设的肌肉途径接种组只获２５％保护，首次证明后海穴具有相对免疫特异性。ＴＧＥ灭活苗后海穴一次接种被动免疫保护率为７７．４５％，主动免疫为９５％。猪流行性腹泻（ＰＥＤ）灭活苗被动免疫保护率为９７．０３％，主动免疫试验０．１ｍｌ及０．５ｍｌ的保护率分别为７７．７８％ｍ和８５％。ＴＧＥ、ＰＥＤ二联灭活苗的被动免疫试验的总保护率ＴＧＥ为８７．９％ＰＥＤ为８２．４％。注苗后攻毒前ＴＧＥ与ＰＥＤ的中和抗体价均为１∶１２８，攻毒后７～１０天中和抗体价均为１∶２５６，主动免疫试验的总保护率：ＴＧＥ为１００％，ＰＥＤ为９７?
points of the stomach channel of Foot Yang Ming and Hou Hai point of Du Mai derian associated with stomach and intestines diseases were inoculated with attenuated transmissible gastroenteritis(TGE)virus.The Hou Hai point was selected to be the best vaccination cupture point with 3/3 protection.Then 4 pregnantwere inoculated at Hou Hai point instead of primary nasal inoculation at the second vaccina sows tion,and protective for piglets was 37/41 with 0 1ml dose,23/38 3 day old piglets were pratected by Hou Hai point route,however,only 1/15 by oral route.Further more,both injection depths of 1 5 cm and 3 0 cm yield 100% protection.Inoculating at 0 5 cm and 1 0 cm away from Hou Hai point display similar protection,the former 4/4 and the later 3/4.On the Contrast,mere 1/4 piglets were protected by intramuscular inoculation route.These results proved that Hou Hai point characterized with a relative speciality on immunity to TGE.The passive and active immune protection for piglets to TGE and porcine epidemic diarrhoea(PED)by Hou Hai point inoculating with inactive vaccine were also evalualed.The inactive TGE vaccine induced 77 45% passive immune protection and 95% active protection.The inactive PED vaccine induced 97 03% passive protection and 77 78% to 85% active protection.The Combinated inactive TGE and PED vaccine showed passive protection 87 98% to TGE and 82 4% to PED,active protection 100% to TGE and 97% to PED.
关键词
猪病
传染性胃肠炎
流行性腹泻
穴位针刺免疫
Transmissible Gastroenteritis
porcine epidemic diarrhoea
Acupture points Vaccination
