摘要
载脂蛋白（a）[apo（a）]免疫BALB／c小鼠，应用杂交瘤技术制备了17株抗apo（a）单克隆抗体（McAb），其中4株与人血清Lp（a）反应良好，且不与低密度脂蛋白（LDL）和纤溶酶原（Pmg）反应。将这4株McAb等量混和作为包被抗体，辣根过氧化物酶（HRP）标记的抗apoB多克隆抗体作为第二抗体，建立了测定人血清Lp（a）的双抗体夹心ELISA法。本法可完全排除apoB和Pmg的干扰，对Lp（a）的特异性良好，有较好的精密度，板内CV为3.8～8.5％，板间CV为4.2～9.6％。以本法测定93例正常人血清Lp（a）的水平，结果表明Lp（a）呈明显的偏态分布，中位数157mg／L，范围为15～1000mg／L。
BALB/c mice were immunized with apo(a) as antigen. 17 hybridomas which secreted monoclonal antidody (McAb) against apo(a) specially were obtained. There were 4 McAb that could well react to not only apo(a) but also Lp(a) and human serum. They could not cross - react with LDL and PMG.The double - antibody sandwich ELISA for determininghuman serum Lp (a) was developed- which coated antitheywas mixture of these 4 McAb and the 2nd antibody was theHRP-labeled anti - apoB polyclonal antithey. This metho dcompletely eliminate the interference of apo(B) and PMG. Ithad good precision, the CV of run - in and run - between was 3. 8 - 8. 5 and 4. 2 - 9. 6, respectivelly. 93 normal human serum Lp(a) were determined, the results indicated that Lp(a) level presented distinct skew distribution, the median was157mg/L and the range was 15 - 1000mg/L.
