摘要
目的分析产PER-1型超广谱β-内酰胺酶(ESBLs)鲍曼不动杆菌的耐药性及其基因的核苷酸序列。方法先后用最低抑菌浓度(MIC)初筛法和纸片扩散确认法从临床分离的鲍曼不动杆菌中检测产ESBLs菌株,并用聚合酶链式反应(PCR)、克隆测序方法分析质粒上PER-1型ESBLs基因。结果93株鲍曼不动杆菌中,产ESBLs菌株有16株,占17.20%,均为多重耐药菌。其中PER-1型ESBLs阳性菌株有8株;TA克隆后测序表明与铜绿假单胞菌(AJ621265.1)blaPER-1基因序列100%同源。结论发现质粒上同时带产TEM-1、PER-1型ESBLs和OXA-23型碳青霉烯酶基因的鲍曼不动杆菌,blaPER-1基因可能来源于铜绿假单胞菌。
[Objective] To analyze drug resistance of Acinetobacter baumannii producing PER-1 ESBLs and its nucleotide sequence. [Methods] The ESBLs producing strains were screened by minimal inhibitory concentration （MIC）method and confirmed by a disc dilution confirmatory test. PER-1 ESBLs gene in plasmid was analyzed by PCR, cloning and nucleotlde sequencing. [Results] There were 16 ESBLs producing strains in 93 isolates of A.bau- mannii and accounted for 17.20%. They were all multi-drug resistance in which 8 were PER-1 ESBLs（＋）. Sequence alignment analysis of this product revealed total identity with blaPER-1 which was originally detected in a strain of Pseudomonas aeruginosa （AJ621265.1）. [Conclusion] An Acinetobacter baumannii which harbors TEM-1, PER-1 type ESBLs and OXA-23 carbarpenase gene has been detected, blaPER-1 gene may be transmitted from Pseudomonas aeruginosa.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第13期1797-1800,共4页
China Journal of Modern Medicine
基金
江西省教育厅课题(No:200693)
