摘要
目的:建立测定胆康片中大黄素、大黄酚含量的HPLC方法。方法:色谱柱为Waters ODS C_(18)(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(85:15),流速为1.0 ml·min^(-1),检测波长为254 nm。结果:大黄素的线性范围为0.03～0.21μg(r=0.999 9)、大黄酚的线性范围为0.06～0.50μg(r=0.999 8),大黄素平均回收率为97.9%、RSD为0.2%(n=6),大黄酚平均回收率为97.6%、RSD为0.4%(n=6)。结论:建立的方法简便、重复性好,可用于胆康片中大黄素、大黄酚的含量测定。
Objective： To establish an HPLC method for determination the contents of emodin and chrysophanol in Dankang Tablets. Method： The Waters ODS C18 column （150 mm × 4.6 mm,5μm）was used,The mobile phase was methanol -0.1% phosphoric acid solution （85：15 ）, the flow rate was 1.0 ml. min - 1. The detection wavelenghth was 254 nm. Result： The linear ranges of emodin and chrysophanol were at 0.03 - 0.21 μg （ r = 0. 999 9 ） and 0.06 - 0.50 μg （ r = 0. 999 8 ）, the average recoveries （ n = 6 ） were 97.9% and 97.6% with RSD 0.2% and 0.4% respectively. Conclusion： The method is simple and repeatable, it can be used for quality control of Dankang tablets.
出处
《中国药师》
CAS
2008年第7期813-814,共2页
China Pharmacist
