摘要
建立了RP-HPLC法测定咖啡中咖啡因和绿原酸的含量,采用ZORBAX SB-C18柱(150mm×4.6mm,5μm),以醋酸缓冲液为流动相,流速为0.8mL·min-1,紫外检测波长为254nm,测定咖啡中咖啡因的含量;以柠檬酸缓冲液和甲醇梯度洗脱(80∶20→50∶50),流速为0.5mL·min-1,紫外检测波长为325nm,测定咖啡中绿原酸含量。结果咖啡因在0.05～0.80mg·mL-1的浓度范围内呈良好的线性关系(r=0.99973),平均回收率(n=6)为99.28%,RSD为3.25%;绿原酸在0.04～0.64mg·mL-1的浓度范围内呈良好的线性关系(r=0.99903),平均回收率为99.39%,RSD为2.73%。
HPLC method for the determination of caffeine and chlorogenic acid in coffee was established. The RP-HPLC method was applied. The analytical column was ZORBAX SB-C18 column （150 mm ×4.6mm,5μm）. For caffeine, the mobile phase was acetic acid buffer solution. The flow rate was 0.8 mL·min-1 and the UV detection was set at 254 nm. For chlorogenic acid, methanol-citric acid buffer solution was adopted as the mobile phase, the proportion was ranged from 20：80 to 50：50. The flow rate was 0.5 mL·min-1 and the UV detection was set at 325 nm. Caffeine showed good linearity （r = 0.999 73） in the range of 0.05-0.80 mg·mL-1. The average recovery （n = 6） was 99.28 % （RSD = 3.25 %）; chlorogenic acid showed good linearity （r = 0.999 03） in the range of 0.04-0.64 mg·mL-1. The average recovery （n = 6） was 99.39 % （RSD = 2.73 %）.
出处
《黑龙江八一农垦大学学报》
2008年第3期64-67,共4页
journal of heilongjiang bayi agricultural university
